Analysis of Gene Expression Differences in the Substrate-Decomposing Ability Degenerated Strains of Agaricus bisporus
文献类型： 会议论文
第一作者： Meiyuan Chen
作者： Meiyuan Chen 1 ; Jianhua Liao 1 ; Zhixin Cai 1 ; Hongrong Li 1 ; Zhongjie Guo 1 ; Zesheng Wang 1 ;
作者机构： 1.Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, China
关键词： Agaricus bisporus;As2796;DDRT-PCR;2-DE;Gene Cloning
会议名称： Congress of the International Society for Mushroom Science
主办单位：
页码： 284-292
摘要： To investigate the gene expression differences and molecular mechanism of substrate-decomposing ability degeneration in Agaricus bisporus (J. E. Lange) Imbach, the strain As2796, cultivated most widely in China, as well as two substrate-decomposing ability degenerated mutants derived from which, 2796-3 and 2796-5, were analyzed by using the techniques of mRNA differential display reverse transcription-PCR ( DDRT-PCR) and two dimensional electrophoresis (2-DE). As a result, five special genes showing obviously differential expressions were found in DDRT-PCR, and some of them have high homologies with substratedecomposing enzymes. Based on the genome sequence data of A. bisporus, PCR primers were designed, and the coding sequences (CDS) with introns and about 1200 bp upstream regulative sequences of above five special genes were amplified, cloned and sequenced, but the comparison of the sequences among three strains showed that the genes and upstream sequences have no repeatable difference. In the 2-DE analysis, two proteins were found to be up-and down-regulated expression, respectively, with obvious and repeatable profiles in both degenerated strains. Based on the results of MALDI-TOF/MS analysis and database searching, these two proteins were identified as an actin and a putative NADH dehydrogenase iron-sulfur protein 3, respectively.
分类号： s646
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