文献类型： 会议论文

第一作者： Zheng Min

作者： Zheng Min ¹ ; Mao Ning ¹ ; Huang Meiqing ¹ ; Chen Shilong ¹ ; Chen Shaoying ¹ ;

作者机构： 1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fujian Animal Diseases Control Technology Development Center,Fuzhou,Fujian

会议名称： International congress on animal hygiene

主办单位：

页码： 225-225

摘要： A pairs of primers and a TaqMan-MCB probes were designed and synthesized according to the the nucleotide sequence of the gE gene of pseudorabies virus (PRV)available in GenBank,and real-time TaqMan-MGB fluorescence quantitative PCR for distinguishing the wild strain and gene-deleted vaccine strain of PRV was established successfully.It was demonstrated that the established TaqMan-MGB quantitative PCR assay could detect 2.23×101 copys·μL-l of plasmid DNA.Sensitivity and positive rate for clinical sample of TaqMan fluorescent quantitative PCR were higher than routine PCR.and its sensitivity was 100 times higher than that of the routine PCR.,and had no cross reaction with classical swine fever virus (CSFV),porcine reproductive and respiratory syndrome virus (PRRSV),porcine cireovirus type 2 (PCV2)and porcine parvovirus (PPV).

分类号： s851.2