科研产出
云南石林乡村旅游规划研究(英文)
《Journal of Landscape Research 》 2011
摘要：Yunnan Province had abundant national cultures,complicated terrains,diversified climates,plentiful biological species,and rich tourist resources.Shilin Yi Autonomous County was located at the central tourist area of Yunnan,was one of the important supporting areas in Central Yunnan tourist region,with large source market.Shilin tourist resources had some advantages,like rich agricultural resources,unique geomorphic feature,and brilliant national culture.There also existed some problems,such as scattered distribution of scenic spots,disordered spatial layout;misuse of spatial resources;representational manifestation of cultural connotation;and monotonous tourist mode.Based on advantages of Shilin tourist resources,the paper had proposed corresponding strategies for tourist planning,that is,① identifying the development thought of self-producing development,national culture driving development,dislocation development and policy linkage,and making rural tourist products with unique style;② adjusting planning layout,integrating tourist resources,forming tourist spatial structure of "one loop and four zones","one loop" referring to tourist loop constituted by shilin Avenue,Jiushi'a travel line and other roads,"four zones" referred to suburb experience zone of "leisure farmhouse",vacation leisure recreational zone,national culture experience zone and outdoor leisure sports zone;③ expanding cultural connotation of rural tourism and creating experience environment for tourists;④ developing multiple tourist modes and meeting diversified needs of source market.It hoped to explore tourist planning more suitable for local tourism development and improve competiveness of local tourist products through perfecting stone forest tourist resources planning.
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缺失免疫抑制位点的人早孕因子重组蛋白的免疫原性
《细胞与分子免疫学杂志 》 2011 北大核心 CSCD
摘要：目的:构建缺失免疫抑制位点的人早孕因子基因的原核表达载体pGEX6P-EPF11,诱导GST-EPF11融合蛋白在大肠杆菌中表达,并免疫小鼠,测定效价。方法:以pMD18T-HSPE1模板,PCR扩增缺失免疫抑制位点的人早孕因子基因片段,经限制性内切酶BamHI和XhoI双酶切后,连接到原核表达载体pGEX-6P-1中,构建重组表达质粒pGEX6P-EPF11。将表达质粒转化大肠杆菌BL21,以1 mmol/L IPTG进行诱导表达GST-EPF11融合蛋白,Western blot鉴定。以表达的GST-EPF11融合蛋白作为免疫原免疫小鼠,抗体效价用ELISA检测。结果:在大肠杆菌中成功表达出相对分子质量约35 000的融合蛋白GST-EPF11。ELISA法检测免疫小鼠的抗体血清可达1∶12 800。结论:在大肠杆菌中成功表达出GST-EPF11融合蛋白,并免疫了小鼠,测定了抗体效价,为下步制备缺失免疫抑制位点的人早孕因子的单克隆抗体(mAb)奠定了基础。
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猪FcγRⅢ和γ链共转染Marc-145细胞系的建立
《华北农学报 》 2011 北大核心 CSCD
摘要：为了建立稳定表达猪FcγRⅢ(Porcine Fc gamma receptorⅢ,poFcγRⅢ)的Marc-145细胞系,从PAM细胞中提取总RNA,应用RT-PCR技术获得猪FcγRⅢ和γ链的cDNA,并构建PIREShyg3-γ和pcDNA3.1-FcγRⅢ真核表达质粒;用脂质体共转染Marc-145细胞,经潮霉素B(300 mg/L)和G418(400 mg/L)共筛选获得稳定表达猪FcγRⅢ的细胞系;运用RT-PCR、玫瑰花环试验和流式细胞术对细胞系进行了鉴定。结果表明,成功构建了猪FcγRⅢ和γ链真核表达载体,建立了稳定表达猪FcγRⅢ的细胞系,表达于转染细胞表面的猪FcγRⅢ受体分子能与猪IgG特异结合。
关键词： 猪FcγRⅢ受体 γ链 共转染 Marc145细胞
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