CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off-Target Evaluation, and Strategies to Mitigate Off-Target Effects

Manghwar, Hakim; Li, Bo; Ding, Xiao; Hussain, Amjad; Zhang, Xianlong; Jin, Shuangxia; Li, Bo; Lindsey, Keith; SCI

机构： Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China; Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Urumqi 830091, Xinjiang, Peoples R China; Univ Durham, Dept Biosci, Durham DH1 3LE, England;

关键词： base editing; CRISPR; Cas systems; off-target effects; PAMs; sgRNA

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Yield sustainability, soil organic carbon sequestration and nutrients balance under long-term combined application of manure and inorganic fertilizers in acidic paddy soil

Qaswar, Muhammad; Jing, Huang; Ahmed, Waqas; Li Dongchu; Liu Shujun; Lu, Zhang; Cai, Andong; Liu Lisheng; Gao Jusheng; Zhang Huimin; Jing, Huang; Li Dongchu; Liu Shujun; Lu, Zhang; Liu Lisheng; Gao Jusheng; Zhang Huimin; Cai, Andong; Xu Yongmei; SCI

机构： Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Natl Engn Lab Improving Qual Arable Land, Beijing 100081, Peoples R China; Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Natl Observat Stn Qiyang Agriecol Syst, Qiyang 426182, Hunan, Peoples R China; Chinese Acad Agr Sci, Key Lab Agroenvironm & Sustainable Dev Agr, Beijing 10081, Peoples R China; Xinjiang Acad Agr Sci, Inst Soil Fertilizer & Agr Water Conservat, Urumqi 830091, Peoples R China;

关键词： Long-term experiment; Carbon sequestration; Yield sustainability; Nutrient balance

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Genome-wide identification and comparative analysis of drought related genes in roots of two maize inbred lines with contrasting drought tolerance by RNA sequencing

Hao Lu-yang; Liu Xu-yang; Zhang Xiao-jing; Zhang Deng-feng; Li Chun-hui; Li Yong-xiang; Shi Yun-su; Song Yan-chun; Wang Tian-yu; Li Yu; Sun Bao-cheng; Liu Cheng; Tang Huai-jun; Xie Xiao-qing; SCI

机构： Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China; Xinjiang Acad Agr Sci, Inst Grain Crops, Urumqi 830002, Peoples R China;

关键词： maize (Zea mays L.); root; transcriptome; RNA sequencing; drought-responsive genes

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Determination of genome size and chromosome ploidy of selected taxa from Prunus armeniaca by flow cytometry

Li, Wenwen; Liu, Liqiang; Wang, Yanan; Liao, Kang; Fan, Guoquan; Zhang, Shikui; Wang, Yatong; SCI

机构： Xinjiang Agr Univ, Coll Hort & Forestry, Urumqi 830052, Xinjiang, Peoples R China; Xinjiang Acad Agr Sci, Luntai Natl Fruit Germplasm Resources Garden, Luntai 841600, Xinjiang, Peoples R China;

关键词： Apricot; 2C DNA content; Ploidy level; Intraspecific variation

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Geographically isolated Colorado potato beetle mediating distinct defense responses in potato is associated with the alteration of gut microbiota

Wang, Jie; Gao, Zhou; Xue, Rongrong; Yan, Hui; Zhang, Zhaojuan; Zeng, Rensen; Yang, Mingyu; Fu, Kaiyun; Guo, Wenchao; Guo, Wenchao; Felton, Gary W.; SCI

机构： Fujian Agr & Forestry Univ, Coll Crop Sci, Minist Educ Genet Breeding & Multiple Utilizat Cr, Key Lab, Fuzhou 350002, Peoples R China; Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China; Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi 830052, Peoples R China; Xinjiang Acad Agr Sci, Inst Microbiol, Xinjiang Lab Special Environm Microbiol, Urumqi 830052, Peoples R China; Penn State Univ, Dept Entomol, University Pk, PA 16802 USA;

关键词： Gut microbiota; Leptinotarsa decemlineata; Invasive insects; Induced defense; Polyphenol oxidase; Solanum tuberosum

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Length-dependent accumulation of double-stranded RNAs in plastids affects RNA interference efficiency in the Colorado potato beetle

He, Wanwan; Xu, Wenbo; Xu, Letian; Bock, Ralph; Zhang, Jiang; Bock, Ralph; Guo, Wenchao; Fu, Kaiyun; SCI

机构： Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China; Max Planck Inst Mol Pflanzenphysiol, Muhlenberg 1, D-14476 Potsdam, Germany; Xinjiang Acad Agr Sci, Inst Microbial Applicat, Urumqi, Peoples R China; Xinjiang Acad Agr Sci, Inst Plant Protect, Urumqi, Peoples R China;

关键词： Colorado potato beetle; double-stranded RNA; pest control; plastid engineering; RNA interference; transplastomic plants

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Field evaluation of sunflower as a potential trap crop of Lygus pratensisin cotton fields

Zhang, Renfu; Wang, Dengyuan; Zhang, Renfu; Wang, Wei; Liu, Haiyang; Yao, Ju; SCI

机构： Xinjiang Agr Univ, Coll Agron, Key Lab Pest Monitoring & Safety Control Crops &, Urumqi, Xinjiang, Peoples R China; Xinjiang Acad Agr Sci, Inst Plant Protect, Key Lab Intergraded Management Harmful Crop Vermi, Minist Agr, Urumqi, Xinjiang, Peoples R China;

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Simulation and Optimization of Venturi Injector by Machine Learning Algorithms

Wang, Haitao; Wang, Jiandong; Wang, Haitao; Yang, Bin; Mo, Yan; Zhang, Yanqun; Ma, Xiaopeng; SCI

机构： Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, 12 South Zhongguancun Rd, Beijing 100081, Peoples R China; China Agr Univ, Coll Water Resources & Civil Engn, 17 East Tsinghua Rd, Beijing 100038, Peoples R China; China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, 20 West Chegongzhuang Rd, Beijing 100048, Peoples R China; Xinjiang Acad Agr Sci, Inst Soil Fertilizer & Agr Water Saving, 403 Nanchang Rd, Urumqi 830091, Peoples R China;

关键词： Venturi injector; Maximum injection rate; Simulation; Machine learning algorithms; Optimal combination

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A Salt Tolerance Evaluation Method for Sunflower (Helianthus annuus L.) at the Seed Germination Stage

Li, Wenhui; Zhang, Huizhen; Zeng, Youling; Li, Tianye; Shen, Fei; Cheng, Quan; Xiang, Lijun; Lei, Zhonghua; Huang, Qixiu; SCI

机构： Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China; Xinjiang Acad Agr Sci, Inst Econ Crops, Urumqi, Peoples R China;

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Comparative transcriptome analysis uncovers different heat stress responses in heat-resistant and heat-sensitive jujube cultivars

Jin, Juan; Yang, Lei; Fan, Dingyu; Hao, Qing; Liu, Xuxin; SCI

机构： Xinjiang Acad Agr Sci, Inst Hort Crops, Urumqi, Peoples R China; Xinjiang Agr Vocat Tech Coll, Changji, Peoples R China;

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