猪繁殖与呼吸综合症GP5蛋白的纯化与免疫活性分析(英文)

文献类型： 中文期刊

第一作者： 陈妍

作者： 陈妍;田宏;尹双辉;尚佑军;刘湘涛

作者机构：

期刊名称： Agricultural Science & Technology

ISSN： 1009-4229

年卷期： 2009 年 03 期

页码： 103-106

摘要： [Objective] The purification and immunocompetence of GPS protein in porcine reproductive and respiratory syndrome (PRRS) were analyzed in this study, which provided basis for establishing the corresponding serological method. [Method] The recombinant expression plasmid pGEX-6P-5 was transformed into BL21 and expressed after being induced with IPTG. The solubility analysis of expression products was carried out, and then the recombinant protein was purified for SDS-PAGE identification and Western-blot analysis. Finally, the recombinant antigen was used in the immune experiment of guinea pigs. [Result] The target protein content accounted for 30% of the total cells protein content according to the chromatography scanning, and the purity of target protein after purification reached 80%. The purified protein was analyzed by Western-blot and immune experiment of guinea pigs, and the results showed that the expressed protein had good reactionogenicity and immunogenicity. [Conclusion] This study provides materials for further studies on the function between PRRSV ORF5 gene and its editing protein, which also lays a foundation for porcine reproductive and respiratory syndrome virus genetic engineering products.

分类号： S858.28

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