以G418为筛选标记的极细链格孢菌(Alternaria tenuissima)原生质体转化体系的建立(英文)

文献类型： 中文期刊

第一作者： 万英

作者： 张大军;黄云;蒋伶活

作者机构：

期刊名称： Agricultural Science & Technology

ISSN： 1009-4229

年卷期： 2008 年 03 期

页码： 65-68+104

摘要： [Objective] The study aimed to establish a protoplast transformation system in Alternaria tenuissima. [Method] The protoplast of A.tenuissima was firstly prepared by enzymolysis method; then the yielded protoplast was transformed by G418 resistant DNA plasmid using PEG/CaCl2 method. [Result] The growth phenotype and PCR detection showed that resistance gene had integrated into A.tenuissima genome. The transformation efficiency of this method reached per μg DNA 3-4 transformants. After subculture thrice under nonselective condition, G418 resistance could still inherit stably. [Conclusion] The transformation system of A.tenuissima was successfully established, which laid basis for studying of the gene function of Alternaria tenuissima.

分类号： S432.4

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