Heat stress response in Chinese cabbage (Brassica rapa L.) revealed by transcriptome and physiological analysis

文献类型： 外文期刊

第一作者： Zhang, Lei

作者： Zhang, Lei;Chen, Guohu;Yuan, Lingyun;Zhu, Shidong;Hou, Jinfeng;Tang, Xiaoyan;Wang, Chenggang;Zhang, Lei;Dai, Yun;Zhang, Shifan;Li, Fei;Zhang, Hui;Li, Guoliang;Zhang, Shujiang;Yue, Lixin

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关键词： Chinese cabbage; Heat stress; Transcriptome; ROS; Physiology

期刊名称：PEERJ （ 影响因子：3.061； 五年影响因子：3.537 ）

ISSN： 2167-8359

年卷期： 2022 年 10 卷

页码：

收录情况： SCI

摘要： High temperatures have a serious impact on the quality and yield of cold-loving Chinese cabbage, which has evolved to have a unique set of stress mechanisms. To explore the relationship between these mechanisms and the heat-tolerance of Chinese cabbage, the physiological indicators of the heat-tolerant ???268??? line and heat-sensitive ???334??? line were measured. Under heat stress, the proline (Pro), soluble sugar (SS), and superoxide dismutase (SOD) indexes of the ???268??? line increased significantly. When additionally using transcriptome analysis, we found that the identified 3,360 DEGs were abundantly enriched in many metabolic pathways including ???plant hormone signal transduction???, ???carbon metabolism???, and ???glycolysis/ gluconeogenesis???. Dynamic gene expression patterns showed that HKL1 in Cluster 15 may be a key factor in the regulation of sugar homeostasis. The interaction network screened four ABA-related genes in Cluster 15, suggesting that high temperatures lead to changes in hormonal signaling, especially an increase in ABA signaling. Compared with the ???334??? line, the expressions of Prx50, Prx52, Prx54, SOD1, and SOD2 in the ???268??? line were significantly upregulated, and these genes were actively involved in the reactive oxygen species (ROS) scavenging process. In summary, our results revealed the relationship between plant heat tolerance, physiology, and biochemistry and may also provide ideas for the future development of high-quality and heat-tolerant Chinese cabbage germplasm resources.

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