Post-transcriptional regulation of grain weight and shape by the RBP-A-J-K complex in rice

文献类型： 外文期刊

第一作者： Ren, Ding

作者： Ren, Ding;Liu, Hui;Sun, Xuejun;Jiang, Ling;Wang, Ying;Jiang, Ning;Yan, Peiwen;Cui, Jinhao;Yang, Jinshui;Lu, Pingli;Luo, Xiaojin;Ren, Ding;Liu, Hui;Sun, Xuejun;Jiang, Ling;Wang, Ying;Jiang, Ning;Yan, Peiwen;Cui, Jinhao;Yang, Jinshui;Lu, Pingli;Luo, Xiaojin;Liu, Hui;Lu, Pingli;Sun, Xuejun;Luo, Xiaojin;Zhang, Fan;Li, Zhikang;Zhang, Fan;Li, Zhikang

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关键词： GL11; grain weight and shape; post-transcriptional regulation; RNA-binding proteins

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2024 年 66 卷 1 期

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收录情况： SCI

摘要： RNA-binding proteins (RBPs) are components of the post-transcriptional regulatory system, but their regulatory effects on complex traits remain unknown. Using an integrated strategy involving map-based cloning, functional characterizations, and transcriptomic and population genomic analyses, we revealed that RBP-K (LOC_Os08g23120), RBP-A (LOC_Os11g41890), and RBP-J (LOC_Os10g33230) encode proteins that form an RBP-A-J-K complex that negatively regulates rice yield-related traits. Examinations of the RBP-A-J-K complex indicated RBP-K functions as a relatively non-specific RBP chaperone that enables RBP-A and RBP-J to function normally. Additionally, RBP-J most likely affects GA pathways, resulting in considerable increases in grain and panicle lengths, but decreases in grain width and thickness. In contrast, RBP-A negatively regulates the expression of genes most likely involved in auxin-regulated pathways controlling cell wall elongation and carbohydrate transport, with substantial effects on the rice grain filling process as well as grain length and weight. Evolutionarily, RBP-K is relatively ancient and highly conserved, whereas RBP-J and RBP-A are more diverse. Thus, the RBP-A-J-K complex may represent a typical functional model for many RBPs and protein complexes that function at transcriptional and post-transcriptional levels in plants and animals for increased functional consistency, efficiency, and versatility, as well as increased evolutionary potential. Our results clearly demonstrate the importance of RBP-mediated post-transcriptional regulation for the diversity of complex traits. Furthermore, rice grain yield and quality may be enhanced by introducing various complete or partial loss-of-function mutations to specific RBP genes using clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 technology and by exploiting desirable natural tri-genic allelic combinations at the loci encoding the components of the RBP-A-J-K complex through marker-assisted selection.

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