Ultrasound-Assisted Extraction, Characterization, and Antioxidant Activities of the Polysaccharides from Fermented Astragalus membranaceus
文献类型： 外文期刊
第一作者： Zhang, Jingyan
作者： Zhang, Jingyan;Liang, Zijing;Zhang, Kang;Tang, Xi;Wang, Lei;Gu, Xueyan;Li, Jianxi;Zhang, Jingyan;Savelkoul, Huub F. J.
作者机构：
关键词：
ultrasound-assisted extraction; polysaccharides from fermented
期刊名称：MOLECULES （ 影响因子：4.6； 五年影响因子：5.0 ）
ISSN：
年卷期： 2025 年 30 卷 5 期
页码：
收录情况： SCI
摘要： This study aimed to optimize the ultrasound-assisted extraction (UAE) of polysaccharides from fermented Astragalus membranaceus (FAPS) and to investigate the physicochemical properties and antioxidant activities of the extracted polysaccharides. Using a combination of single-factor experiments and response surface methodology based on a Box-Behnken design, we improved the extraction of crude FAPS without deproteinization. Under optimal conditions (50 degrees C, 60 min, 8 mL/g, 480 W), the yield of crude FAPS obtained by UAE (7.35% +/- 0.08) exceeded the yield from convectional hot water extraction (6.95% +/- 0.24). After protein removal, the FAPS was subjected to comprehensive chemical analyses, including HPLC, HPGPC, FT-IR, UV spectroscopy, and a Congo red assay. The results showed that FAPS had a significantly higher carbohydrate content compared to the non-fermented group (95.38% +/- 6.20% vs. 90.938% +/- 3.80%), while the protein content was significantly lower than that of the non-fermented Astragalus polysaccharides (APS) group (1.26% +/- 0.34% vs. 6.76% +/- 0.87%). In addition, FAPS had a higher average molecular weight and a lower Mw/Mn ratio compared to APS. The primary monosaccharides in FAPS were identified as Glc, Ara, Gal and GalA, with a molar ratio of 379.72:13.26:7.75:6.78, and FAPS lacked a triple helix structure. In vitro, antioxidant assays showed that FAPS possessed superior antioxidant properties compared to APS. These results emphasize the significant potential of FAPS as an antioxidant, possibly superior to that of APS. The results of this study suggest that fermentation and UAE offer promising applications for the development and utilization of Astragalus membranaceus for human and animal health.
分类号：
- 相关文献
作者其他论文 更多>>
-
Metabolic profiling of Perilla leaves of different colors: insights into metabolite variation and bioactive compound distribution
作者：Dossou, Senouwa Segla Koffi;Deng, Qianchun;Wang, Lei;Kefale, Habtamu;Zhou, Rong;Li, Donghua;Tan, Meilian;Wang, Linhai;Dossou, Senouwa Segla Koffi;Li, Feng;Jiang, Nanjun
关键词：Perilla; Metabolite profiling; Leaf color; Differentially accumulated metabolites; LC-MS/MS
-
Fish-derived cationic antimicrobial peptide piscidin 1 exerts broad-spectrum bactericidal activities against multidrug-resistant Gram-negative pathogens
作者：Sun, Huarun;Zhang, Jingao;Li, Xiaolu;Gao, Haolei;Liao, Chengshui;Wang, Ruibiao;Wen, Bo;Luo, Weiyu;Zhang, Wei;Zhu, Chunling;Wen, Yuliang;Zhang, Longfei;Liu, Xuehan;Shen, Jiyuan;Hu, Jianhe;Bai, Yueyu;Zhang, Huihui;Ding, Ke;Wang, Lei;Sun, Huarun;Zhang, Jingao;Gao, Haolei;Wang, Ruibiao;Wen, Bo;Luo, Weiyu;Zhang, Wei;Zhu, Chunling;Wen, Yuliang;Zhang, Longfei;Liu, Xuehan;Shen, Jiyuan;Hu, Jianhe;Zhang, Huihui;Wang, Lei;Liao, Chengshui;Ding, Ke;Wu, Yundi;Wu, Xilong;Li, Hao
关键词：Antimicrobial peptide; Multiresistance; Piscidin 1; Bactericidal mechanism; Gram-negative bacteria
-
Soybean yield estimation and lodging classification based on UAV multi-source data and self-supervised contrastive learning
作者：Zhou, Longyu;Chen, Haochong;Feng, Puyu;Ma, Yuntao;Zhang, Yong;Wang, Lei;Li, Mingxue;Sun, Xuhong;Sun, Guangyao;Yan, Long;Qiu, Lijuan;Li, Yinghui
关键词：Remote sensing; 3D reconstruction; Point cloud; Multispectral; Structure from motion
-
The transcription factor CCT30 promotes rice preharvest sprouting by regulating sugar signalling to inhibit the ABA-mediated pathway
作者：Fan, Xiaowei;Liu, Yuexin;Huang, Wen;Yang, Ying;Zhang, Jia;Qi, Feixiang;Wang, Lei;Song, Song;Xing, Yongzhong;Fan, Xiaowei;Song, Song;Gao, Fangyuan;Luo, Zhengliang;Lv, Jianqun;Su, Xiangwen;Ren, Guangjun;Xing, Yongzhong
关键词：preharvest sprouting; CCT30; OsbZIP37; ABA-mediated pathway; sugar signalling
-
Population sequencing of cherry accessions unravels the evolution of Cerasus species and the selection of genetic characteristics in edible cherries
作者：Lei, Yahui;Jiu, Songtao;Xu, Yan;Lv, Zhengxin;Liu, Xunju;Wang, Lei;Wang, Li;Wang, Jiyuan;Zhang, Zhuo;Wang, Shiping;Zhang, Caixi;Lei, Yahui;Dong, Xiao;Dong, Yang;Chen, Baozheng;Dong, Yang;Bernard, Anthony;Barreneche, Teresa;Elisabeth, Dirlewanger;Cai, Yuliang;Zheng, Wei;Zhang, Xu;Li, Fangdong;Li, Hongwen;Liu, Congli;Li, Ming;Wang, Jing;Zhu, Jijun;Peng, Lei;Yu, Fei;Duan, Shengchang
关键词：
Cerasus subgenus; Population structure; Genetic diversity; Edible cherries; Selection signatures -
Zn2+inhibits PEDV replication by inducing autophagy through the Akt-mTOR pathway
作者：Wang, Caiying;Zhang, Yue;Liu, Guangliang;Wang, Caiying;Yang, Shanshan;Savelkoul, Huub F. J.;Jansen, Christine A.
关键词：Zinc; PEDV; Autophagy; VPS34; Akt-mTOR
-
Bridging the gap between hyperspectral imaging and crop breeding: soybean yield prediction and lodging classification with prototype contrastive learning
作者：Sun, Guangyao;Han, Shiteng;Xu, Yun;Ma, Yuntao;Zhang, Yong;Wang, Lei;Zhou, Longyu;Fei, Shuaipeng;Xiao, Shunfu;Ma, Yuntao;Yan, Long;Che, Yingpu;Li, Yinghui;Qiu, Lijuan
关键词：Unmanned Aerial Vehicle; Deep Learning; Resnet; Soybean Yield and Lodging Prediction
