Establishment and characterization of a cell line from Yunlong grouper (Epinephelus moara ♀ and Epinephelus lanceolatus ♂) larva and its response to infectious spleen and kidney necrosis virus infection

文献类型： 外文期刊

第一作者： Guo, Chenfei

作者： Guo, Chenfei;Sha, Zhenxia;Guo, Chenfei;Gong, Zhihong;Fang, Fei;Zhang, Qi;Wang, Na;Su, Yuqin;Wang, Lei;Chen, Songlin;Chen, Songlin

作者机构：

关键词： Yunlong grouper; Larvae; Cell line; Iridovirus; Glar

期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）

ISSN： 0044-8486

年卷期： 2025 年 606 卷

页码：

收录情况： SCI

摘要： The Yunlong grouper is a novel hybrid species resulting from the crossbreeding of Epinephelus moara female and Epinephelus lanceolatus male. This hybrid species exhibits a combination of desirable traits inherited from both parent species. This study established the first Yunlong grouper cell line from larvae, named Glar (Grouper larvae). The cells were cultured in L-15 medium containing 15 % serum in 24 degrees C and have culture more than 80 passages. Chromosome karyotype analysis showed that the chromosome number was 2n = 48, and transcriptome analysis indicated that the cell line is mainly composed of fibroblast. After cryopreservation in liquid nitrogen for 6 months, cell viability remained above 70 %. The cells can be transfected, as fluorescence was observed after transfection with both Cy3-labeled scramble siRNA and the pEGFP-N1 plasmid. Furthermore, after infection with two iridoviruses, spotted knifejaw iridovirus (SKIV-SD) and spotted seabass iridovirus (SBIV-V12), Glar cells showed significant cytopathic effect (CPE), and viral particles were observed using transmission electron microscopy. Transcriptome analysis revealed that five days post-infection with SBIV-V12, differentially expressed genes were primarily enriched in pathways related to cell replication, the cell cycle, DNA repair, the p53 signaling pathway, and the Fanconi anaemia pathway, with 20 of 21 genes in the p53 pathway showing upregulation. The p53 signaling pathway plays a crucial role in antiviral immunity. Therefore, this cell line can serve as a valuable tool for in vitro research on the prevention and control of iridovirus, as well as for studying the disease mechanisms and potential therapeutic strategies for the Yunlong grouper.

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