Knockdown of tyrosine hydroxylase gene affects larval survival, pupation and adult eclosion in Plagiodera versicolora

文献类型： 外文期刊

第一作者： Liu, Xiaolong

作者： Liu, Xiaolong;Wang, Xin;Zhang, Qi;Lu, Min;Ze, Longji;Zhang, Hainan

作者机构：

关键词： cuticle tanning; mortality; Plagiodera versicolora; RNA interference; tyrosine hydroxylase

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：2.3； 五年影响因子：2.9 ）

ISSN： 0962-1075

年卷期： 2025 年 34 卷 2 期

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收录情况： SCI

摘要： In insects, tyrosine hydroxylase (TH) plays essential roles in cuticle tanning and cuticle pigmentation. Plagiodera versicolora (Coleoptera: Chrysomelidae) is a leaf-eating forest pest in salicaceous trees worldwide. However, the function of PverTH in P. versicolora is still unknown. In this study, we obtained a PverTH gene from transcriptome analysis. The expression analysis of PverTH showed that the highest expression was found in epidermis of larvae. In this study, we used RNA interference (RNAi) technology to knockdown the PverTH gene. The results showed that ingestion of dsTH led to cuticle coloration became lighter in larvae, pupae and adults. Knockdown of PverTH gene inhibited larval growth, and consequently caused higher mortality. In addition, RNAi of TH disrupted the cuticle tanning, caused lower pupation rate, lower eclosion rate and higher deformity rate. This study indicates that PverTH is vital for the cuticular pigments and cuticle tanning. Moreover, this research suggested that the development of PverTH gene as a potential target gene to control P. versicolora.

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