Piperine Derived from Piper nigrum L. Inhibits LPS-Induced Inflammatory through the MAPK and NF-kappa B Signalling Pathways in RAW264.7 Cells

文献类型： 外文期刊

第一作者： Duan, Zhouwei

作者： Duan, Zhouwei;Yang, Hong;Duan, Zhouwei;Xie, Hui;Yu, Shasha;Wang, Shiping;Yu, Shasha

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关键词： Piper nigrum L.; anti-inflammatory; piperine; MAPK; NF-kappa B

期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）

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年卷期： 2022 年 11 卷 19 期

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收录情况： SCI

摘要： Piperine, an important natural product, has a good anti-inflammatory effect. However, few researchers have studied its mechanism in these pathways. The objective of this research was to evaluate the molecular mechanism underlying the anti-inflammatory responses of piperine in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The purification and characterization of piperine from Piper nigrum L. were determined by HPLC, UPLC-Q-TOF-MS and H-1 NMR. Then, the anti-inflammatory activity was evaluated by a reagent test kit, ELISA kits, RT-PCR and Western blot experiments. The results suggested that piperine (90.65 +/- 0.46% purity) at a concentration of 10-20 mg/L attenuated the production of NO and ROS, downregulated the protein and mRNA expression levels of TNF-alpha, IL-1 beta and IL-6, and upregulated the protein and mRNA transcription levels of IL-10. Meanwhile, the Western blot results indicated that piperine could inhibit the phosphorylation levels of the ERK, JNK, p38 and p65 proteins. Our findings suggest that piperine is a potential anti-inflammatory substance, whose molecular mechanism may be to regulate the key factors of the NF-kappa B and MAPK signalling pathways.

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