The CsPbs2-interacting protein oxalate decarboxylase CsOxdC3 modulates morphosporogenesis, virulence, and fungicide resistance in Colletotrichum siamense

文献类型： 外文期刊

第一作者： Lu, Jingwen

作者： Lu, Jingwen;Liu, Yu;Song, Miao;Xi, Yitao;Yang, Hong;Liu, Wenbo;Li, Xiao;Norvienyeku, Justice;Zhang, Yu;Miao, Weiguo;Lin, Chunhua;Yang, Hong

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关键词： Colletotrichum siamense; HOG MAPK pathway; Oxalate decarboxylase; Stress homeostasis; Fungicide sensitivity; Virulence

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.7； 五年影响因子：7.1 ）

ISSN： 0944-5013

年卷期： 2024 年 284 卷

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收录情况： SCI

摘要： The HOG MAPK pathway mediates diverse cellular and physiological processes, including osmoregulation and fungicide sensitivity, in phytopathogenic fungi. However, the molecular mechanisms underlying HOG MAPK pathway-associated stress homeostasis and pathophysiological developmental events are poorly understood. Here, we demonstrated that the oxalate decarboxylase CsOxdC3 in Colletotrichum siamense interacts with the protein kinase kinase CsPbs2, a component of the HOG MAPK pathway. The expression of the CsOxdC3 gene was significantly suppressed in response to phenylpyrrole and tebuconazole fungicide treatments, while that of CsPbs2 was upregulated by phenylpyrrole and not affected by tebuconazole. We showed that targeted gene deletion of CsOxdC3 suppressed mycelial growth, reduced conidial length, and triggered a marginal reduction in the sporulation characteristics of the Delta CsOxdC3 strains. Interestingly, the Delta CsOxdC3 strain was significantly sensitive to fungicides, including phenylpyrrole and tebuconazole, while the CsPbs2 -defective strain was sensitive to tebuconazole but resistant to phenylpyrrole. Additionally, infection assessment revealed a significant reduction in the virulence of the Delta CsOxdC3 strains when inoculated on the leaves of rubber tree ( Hevea brasiliensis ). From these observations, we inferred that CsOxdC3 crucially modulates HOG MAPK pathway-dependent processes, including morphogenesis, stress homeostasis, fungicide resistance, and virulence, in C. siamense by facilitating direct physical interactions with CsPbs2. This study provides insights into the molecular regulators of the HOG MAPK pathway and underscores the potential of deploying OxdCs as potent targets for developing fungicides.

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