High-throughput screening and investigation of the inhibitory mechanism of alpha-glucosidase inhibitors in teas using an affinity selection-mass spectrometry method

文献类型： 外文期刊

第一作者： Gao, Jianjian

作者： Gao, Jianjian;Zhou, Mengxue;Chen, Dan;Xu, Jiye;Wang, Zhe;Peng, Jiakun;Lin, Zhiyuan;Yu, Shuai;Lin, Zhi;Dai, Weidong;Gao, Jianjian;Xu, Jiye;Peng, Jiakun;Yu, Shuai

作者机构：

关键词： Tea; Galloylated polyphenol; Affinity selection-mass spectrometry; alpha-Glucosidase inhibitor; High-throughput screening; Inhibitory mechanism

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 422 卷

页码：

收录情况： SCI

摘要： An affinity selection-mass spectrometry method was applied for high-throughput screening of a-glucosidase (AGH) inhibitors from teas. Fourteen out of nineteen screened AGH inhibitor candidates were clustered as galloylated polyphenols (GPs). "AGH-GPs" interaction studies, including enzyme kinetics, fluorescence spec-troscopy, circular dichroism, and molecular docking, jointly suggested that GPs noncompetitively inhibit AGH activity by interacting with amino acid residues near the active site of AGH and inducing changes in AGH secondary structure. Representative GPs and white tea extract (WTE) showed comparable AGH inhibition effects in Caco2 cells and postprandial hypoglycemic efficacy in diabetic mice as acarbose. The area under the curve of oral sucrose tolerance test was lower by 8.16%, 6.17%, and 7.37% than control group in 15 mg/kg EGCG, 15 mg/ kg strictinin, and 150 mg/kg WTE group, respectively. Our study presents a high-efficiency approach to discover novel AGH inhibitors and elucidates a potential mechanism by which tea decreases diabetes risks.

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