Establishment and application of quantitative detection of Bacillus velezensis HMB26553, a biocontrol agent against cotton damping-off caused by Rhizoctonia
文献类型： 外文期刊
第一作者： Su, Zhenhe
作者： Su, Zhenhe;Liu, Gaoge;Li, Cong;Liu, Xiaomeng;Guo, Qinggang;Wang, Peipei;Dong, Lihong;Lu, Xiuyun;Zhao, Weisong;Zhang, Xiaoyun;Qu, Yuanghang;Zhang, Jiaqi;Mo, Shaojing;Li, Shezeng;Ma, Ping;Ma, Ping
作者机构：
关键词： Bacillus velezensis; detection; quantification; strain-specific primers; TaqMan qPCR; unique gene pB-gene0026
期刊名称：BIOTECHNOLOGY JOURNAL （ 影响因子：4.7； 五年影响因子：4.5 ）
ISSN： 1860-6768
年卷期： 2024 年 19 卷 2 期
页码：
收录情况： SCI
摘要： A highly sensitive quantitative PCR (qPCR) method was developed for detection and quantification of Bacillus velezensis HMB26553 in cotton rhizosphere. The study aimed to develop a quantitative detection method for the strain HMB26553, and explore the relationship between its colonization of the cotton rhizosphere and its control effect. The whole genome sequence of strain HMB26553 was obtained by genome sequencing and a unique specific sequence pB-gene0026 on plasmid plaBV2 was identified by using high-throughput alignment against NCBI. Plasmid plaBV2 could be stably genetically inherited. Based on this sequence, specific primers for amplifying 106 bp and a minor groove binder (MGB) TaqMan probe for enhancing sensitivity were designed. The copy number of plaBV2 in strain HMB26553, which was 2, was confirmed by internal reference primers and the MGB TaqMan probe based on housekeeping gene gyrB. The established detection technique based on these primers and probes had high specificity and sensitivity compared to traditional plate counting method, with a detection limit of 1.5 copy genome. Using this method, the study discovered a likely correlation between the quantity of colonization in cotton rhizosphere and efficacy against cotton damping-off caused by Rhizoctonia after seed soaking and irrigation with strain HMB26553. Thus, this method provides scientific support for the rational application of strain HMB26553 in the future.
分类号：
- 相关文献
作者其他论文 更多>>
-
Microbial Community Structure and Diversity of Endophytic Bacteria and Fungi in the Healthy and Diseased Roots of Angelica sinensis, and Identification of Pathogens Causing Root Rot
作者：Cheng, Yaya;Zhang, Xiaoyun;Zhang, Wenwen;Dong, Jianmei;Ma, Yanjun;Zhang, Aimei;Kong, Weibao;Han, Fujun;Peng, Hai;Kong, Weibao
关键词：
Angelica sinensis ; endophytes; microbial community structure; diversity analysis; root rot -
Wheat TaPYL9-involved signalling pathway impacts plant drought response through regulating distinct osmotic stress-associated physiological indices
作者：Zhang, Yanyang;Zhao, Yingjia;Hou, Xiaoyang;Zhang, Chunlin;Wang, Ziyi;Zhang, Jiaqi;Liu, Xianchang;Shi, Xinxin;Duan, Wanrong;Xiao, Kai;Zhang, Yanyang;Zhao, Yingjia;Hou, Xiaoyang;Zhang, Chunlin;Wang, Ziyi;Zhang, Jiaqi;Liu, Xianchang;Shi, Xinxin;Duan, Wanrong;Xiao, Kai;Zhao, Yingjia
关键词：wheat (
Triticum aestivum L.); abscisic acid signalling pathway; drought stress; gene transcription and function; protein-protein interaction assay -
Accumulation of Water-Soluble Polysaccharides during Lychee Pulp Fermentation with Lactiplantibacillus plantarum Involves Endoglucanase Expression
作者：Huang, Guitao;Zou, Xiaoqin;Dong, Lihong;Deng, Mei;Zhang, Ruifen;Huang, Fei;Zhang, Mingwei;Huang, Guitao;Zhang, Mingwei;Su, Dongxiao;Lee, Yuan-kun
关键词：lychee; polysaccharides; Lactobacillus; endoglucanase; galactosidase
-
Farmers' decisions on crop residues utilization, greenhouse gases reduction and subsidy of crop residue-based bioenergy: An agent-based life cycle model
作者：Zhang, Jiaqi;Zhang, Jiaqi;Wang, Bin;Li, Yu'e;Zhang, Jiaqi;Oosterveer, Peter;Zhuge, Chengxiang;Huang, Qitong;Huang, Qitong
关键词：Agent-based model; Crop residue-based bioenergy; Life cycle thinking; Environmental and economic impacts; Farmer decision making
-
Suppressiveness of spent mushroom substrate amendment against eggplant Verticillium wilt
作者：Qu, Yuanhang;Qu, Yuanhang;Liu, Xiaomeng;Su, Zhenhe;Guo, Qinggang;Ma, Ping
关键词：eggplant Verticillium wilt; spent mushroom substrate; rhizosphere;
Bacillus ; soil amendment -
Genetic and Functional Dissection of the NFKB2 Gene: Implications for Milk Fatty Acid Biosynthesis in Dairy Cattle
作者：Shi, Lijun;Guo, Zijiao;Shen, Nan;Li, Cong;Han, Bo;Sun, Dongxiao;Shi, Lijun
关键词：dairy cattle; EMSA; fatty acids; knocked-down of
NFKB2 ; transcriptional activity -
A trans-long-chain prenyl diphosphate synthase promotes ubiquinone 10 biosynthesis in grape
作者：Wang, Peipei;Ren, Tingting;You, Ruyue;Liu, Yuting;Ji, Xinglong;Ren, Yiran;Leng, Xiangpeng;Ren, Tingting;You, Ruyue;Ji, Xinglong;Ren, Yiran;Leng, Xiangpeng;Cao, Yazheng;Xu, Yang;Wei, Guo;Zhang, Kekun;Zhou, Yongfeng;Zhou, Yongfeng;Sun, Lei
关键词：
