Microbial Community Structure and Diversity of Endophytic Bacteria and Fungi in the Healthy and Diseased Roots of Angelica sinensis, and Identification of Pathogens Causing Root Rot

文献类型： 外文期刊

第一作者： Cheng, Yaya

作者： Cheng, Yaya;Zhang, Xiaoyun;Zhang, Wenwen;Dong, Jianmei;Ma, Yanjun;Zhang, Aimei;Kong, Weibao;Han, Fujun;Peng, Hai;Kong, Weibao

作者机构：

关键词： Angelica sinensis; endophytes; microbial community structure; diversity analysis; root rot

期刊名称：MICROORGANISMS （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN：

年卷期： 2025 年 13 卷 2 期

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收录情况： SCI

摘要： Angelica sinensis (Oliv.) Diels is an important traditional Chinese herbal medicine, and its main medicinal part is the root. In recent years, root rot has become one of the bottlenecks hindering the healthy and green development of Angelica cultivation due to the inappropriate application of chemical fertilizers, pesticides, plant growth regulators, and continuous cropping. In this study, high-throughput sequencing technology was adopted to reveal the differences in the community structure and diversity of endophytic bacteria and fungi in the roots of healthy and diseased A. sinensis. The results showed that the diversity index of endophytic bacterial communities was significantly higher in healthy root than in diseased Angelica root systems. There was a significant difference in endophytic fungal community diversity only at the m1 sampling site. There was a significant difference in the beta-diversity of bacterial communities, but not of fungi. In terms of community composition, Proteobacteria was the dominant phylum of bacteria, and Sphingobium and Pseudomonas were the dominant genera; Ascomycota and Basidiomycota were the dominant phyla of fungi, and Plectosphaerella, Paraphoma, and Fusarium were the dominant genera. In addition, the relative abundance of the genera Sphingobium and Pseudomonas was higher in healthy roots, while Fusarium was higher in diseased samples. Among the five pathogens isolated from diseased root, four strains were Fusarium sp., and one was Paraphoma chrysanthemicola, which is reported for the first time. Our findings indicate that the endophyte community structure of A. sinensis infected with root rot changed significantly compared with healthy plants, and Fusarium is an important pathogenic factor, which provides a valuable microbiological basis for the targeted biocontrol of Angelica root rot.

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