Strain prioritization for lipopeptide-producing bacteria with antagonistic effects against Fusarium graminearum

文献类型： 外文期刊

第一作者： Wang, Gang

作者： Wang, Gang;Wang, Yuting;Chen, Daoming;Liu, Xin;Shi, Jianrong;Xu, Jianhong;Wang, Gang;Wang, Yuting;Xu, Jianhong;Wang, Gang;Zhang, Xiaoyun;Xu, Jianhong;Hu, Junqiang;Mohamed, Sherif Ramzy;Lee, Yin-Won;Yang, Chenye

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关键词： Strain prioritization; Biocontrol; Fusarium graminearum; lipopeptides; Bacillus amyloliquefaciens; antagonistic mechanism

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2025 年 206 卷

页码：

收录情况： SCI

摘要： The phytopathogenic fungus Fusarium graminearum causes severe plant disease such as wheat scab and poses threats to human health by mycotoxin contamination. Green agriculture's rise has brought growing attention to biological control methods, which contributes to pesticide reduction. In this study, we employed a quantitative reverse transcription PCR (qRT-PCR) based high-throughput screening method to identify bacterial strains that produce lipopeptides. By using this method, we identified Bacillus amyloliquefaciens strain 7D3, which exhibits high lipopeptide production and strong antifungal activity. The crude extract of 7D3 fermentation broth exhibited an EC50 of 75.09 +/- 6.72 mu g/mL. Analysis of the metabolites via liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) revealed numerous lipopeptides, such as surfactin and iturin. The strain inhibited Fusarium graminearum infection by disrupting the cell membrane, thus damaging the mycelial structure of the fungus and resulting in disordered hyphal osmotic characteristics and eventually hyphal rupture. This strain also inhibited sporulation as well as germination of Fusarium spores. Additionally, greenhouse trials demonstrated that the 7D3 strain effectively suppressed Fusarium infection in wheat while also inhibiting the accumulation of mycotoxins. This study provides an alternative method for screening biocontrol agents with activity against FHB.

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