Dynamic visualization of DNA methylation in cell cycle genes during iPSC cardiac differentiation

文献类型： 外文期刊

第一作者： Li, Ning

作者： Li, Ning;Zhang, Zhenhe;Maclellan, W. Robb;Zhang, Yiqiang;Li, Ning;Nguyen, Ba Thong;Stitt, Edward A.;Zhang, Yiqiang;Nguyen, Ba Thong;Zhang, Yiqiang;Stitt, Edward A.;Zhang, Yiqiang

作者机构：

关键词： Cardiac differentiation; cell cycle; DNA methylation; epigenetics; GMR (genomic DNA methylation reporter); pyrosequencing; iPSC (induced pluripotent stem cell); single-cell imaging

期刊名称：EPIGENOMICS （ 影响因子：2.6； 五年影响因子：2.8 ）

ISSN： 1750-1911

年卷期： 2024 年 16 卷 23-24 期

页码：

收录情况： SCI

摘要： BackgroundExisting analyses with conventional assays have generated significant insights into static states of DNA methylation but were unable to visualize the dynamics of epigenetic regulation.Materials & ResultsWe utilized a genomic DNA methylation reporter (GMR) system carrying Snrpn minimal promoter and CpG regions of Cdk1 (Cyclin-dependent kinase 1) or Sox2 (SRY-Box Transcription Factor 2). Mouse Sox2 GMR iPSCs rapidly lost fluorescent reporter signal upon the induction of cardiac differentiation. Cdk1 GMR reporter signal was strong in undifferentiated iPSCs, and gradually decreased during cardiomyocyte differentiation. RT-qPCR and pyrosequencing demonstrated that the reduction of Sox2 and Cdk1 was regulated by hypermethylation of their promoters' CpG regions during cardiac differentiation.ConclusionThe GMR reporter system can be useful for monitoring real-time epigenetic DNA modification at single-cell resolution.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序