Intestinal inflammation disrupts energy metabolism in layer pullets: insights into energy partitioning and intestinal metabolomic profiling

文献类型： 外文期刊

第一作者： Jiang, Qiuyu

作者： Jiang, Qiuyu;Huang, Bingjian;Zhao, Lihua;Zhang, Bingkun;Chen, Simiao;Ban, Zhibin

作者机构：

关键词： Adenosine triphosphate; Dextran sulfate sodium; Heat production; Inflammation; Metabolomic

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：6.5； 五年影响因子：7.2 ）

ISSN： 1674-9782

年卷期： 2025 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Background Intestinal inflammation is an energy-consuming process that may alter energy supply and demand in poultry. During inflammation, the intestinal energy metabolic profile and the patterns of energy partitioning remain unclear. This study investigated the effects of intestinal inflammation on energy intake, heat production (HP), retained energy (RE) and intestinal energy metabolites in layer pullets. Methods After 7 d dietary adaption, 32 "Jing Tint 6" layer pullets with average body weight (1,123.50 +/- 8.55 g) were selected from 96 birds, and randomly assigned to two groups (CON: Control group, INFL: Inflammation group) with 8 replicates per group. Indirect calorimetry analysis was conducted over 7 d to determine HP and fasting HP (FHP). During this period, pullets in INFL group received 4 mL/d of 0.6 g/mL dextran sulfate sodium (DSS) via oral gavage to induce intestinal inflammation. After the calorimetry, intestinal tissues were collected post-euthanasia from one bird per replicate for morphological and mucosal metabolomic analysis. Results Birds exhibited significantly lower apparent metabolizable energy (AME) intake (P < 0.001) during intestinal inflammation, accompanied by compromised RE and RE as fat (P < 0.001), suggesting that birds consumed body energy to sustain energy demands. Targeted metabolomic studies identified 11 energy metabolites differentially expressed in ileal mucosa between CON and INFL groups. Specifically, DSS induction significantly increased (P < 0.05) adenosine triphosphate (ATP) level and reduced (P < 0.001) nicotinamide adenine dinucleotide (NAD+) level in ileal mucosa of pullets. In parallel, metabolic adaptations such as enhanced glycolytic intermediates, reduced amino acids, alpha-ketoglutarate (alpha-KG) accumulation and suppressed expression of genes encoding enzymes involved in tricarboxylic acid (TCA) cycle were observed in the inflamed ileum of pullets. Conclusion Immune stimulation by DSS induced a negative energy balance in layer pullets, characterized by reduced AME intake (-190.47 kJ/kg BW0.75) and compromised RE (-18.81% of AME intake). Disruption of intestinal energy profiling was observed in inflammation-challenged pullets, such as accumulation of alpha-KG and ATP, reduced NAD+ and amino acids, which could provide valuable insights for developing effective intervention strategies.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序