Regulatory Mechanism of the GmMYB14 Transcription Factor on Auxin-Related Proteins in Soybean

文献类型： 外文期刊

第一作者： Peng, Lihua

作者： Peng, Lihua;Liu, Yangyan;Yang, Hongli;Guo, Wei;Hao, Qingnan;Chen, Shuilian;Yuan, Songli;Zhang, Chanjuan;Yang, Zhonglu;Han, Bei;Huang, Yi;Shan, Zhihui;Chen, Limiao;Chen, Haifeng;Peng, Lihua;Liu, Yangyan

作者机构：

关键词： soybean; plant architecture; MYB transcription factor; indole-3-acetic acid (IAA); GmIAA1

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 16 期

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收录情况： SCI

摘要： In a previous study, GmMYB14 overexpressing (GmMYB14-OX) transgenic soybean plants displayed a semi-dwarfism and compact phenotype, which was regulated by the brassinosteroid (BR) pathway. However, the phenotype of GmMYB14-OX plants could be partly rescued after spraying them with exogenous BR. This indicates that other hormones, in addition to BR, also play a role in regulating the architecture of GmMYB14-OX plants. We observed a significant decrease in the content of endogenous indole-3-acetic acid (IAA) in transgenic soybean lines (OX9 and OX12) compared to wild type (WT) plants. The plant height, leaf area, leaf petiole length, and leaf petiole angle of GmMYB14-OX plants could also be partly rescued after applying exogenous IAA for two weeks. Transcriptome sequencing analysis revealed that the expression of many genes within the Aux/IAA gene family underwent alterations in the GmMYB14-OX transgenic soybean plants. Among them, Glyma.02G000500 (GmIAA1) showed the highest expression in GmMYB14-OX plants. Furthermore, the results of electrophoretic mobility shift assay and dual-luciferase reporter indicate that GmMYB14 protein could bind to the promoter of GmIAA1. In summary, a decrease in endogenous IAA content may be one of the factors contributing to the compact and dwarfed architecture of GmMYB14-OX plants. GmMYB14 also acts as a transcriptional activator of GmIAA1 to potentially block IAA effects. Our findings provide a theoretical basis for further investigation of the regulatory mechanism of GmMYB14 on soybean plant architecture.

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