Verticillium dahliae CFEM proteins manipulate host immunity and differentially contribute to virulence

文献类型： 外文期刊

第一作者： Wang, Dan

作者： Wang, Dan;Zhang, Dan-Dan;Song, Jian;Li, Jun-Jiao;Wang, Jun;Li, Ran;Kong, Zhi-Qiang;Dai, Xiao-Feng;Chen, Jie-Yin;Klosterman, Steven J.;Lin, Fa-Zhuang;Subbarao, Krishna V.

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关键词： Verticillium dahliae; CFEM domain; Function divergence; Suppress immunity; Iron response

期刊名称：BMC BIOLOGY （ 影响因子：7.364； 五年影响因子：8.641 ）

ISSN：

年卷期： 2022 年 20 卷 1 期

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收录情况： SCI

摘要： Background Verticillium dahliae is a fungal pathogen that causes a vascular wilt on many economically important crops. Common fungal extracellular membrane (CFEM) domain proteins including secreted types have been implicated in virulence, but their roles in this pathogen are still unknown. Results Nine secreted small cysteine-rich proteins (VdSCPs) with CFEM domains were identified by bioinformatic analyses and their differential suppression of host immune responses were evaluated. Two of these proteins, VdSCP76 and VdSCP77, localized to the plant plasma membrane owing to their signal peptides and mediated broad-spectrum suppression of all immune responses induced by typical effectors. Deletion of either VdSCP76 or VdSCP77 significantly reduced the virulence of V. dahliae on cotton. Furthermore, VdSCP76 and VdSCP77 suppressed host immunity through the potential iron binding site conserved in CFEM family members, characterized by an aspartic acid residue in seven VdSCPs (Asp-type) in contrast with an asparagine residue (Asn-type) in VdSCP76 and VdSCP77. V. dahliae isolates carrying the Asn-type CFEM members were more virulent on cotton than those carrying the Asp-type. Conclusions In the iron-insufficient xylem, V. dahliae is likely to employ the Asp-type CFEM members to chelate iron, and Asn-type CFEM members to suppress immunity, for successful colonization and propagation in host plants.

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