An Importin beta Protein Negatively Regulates MicroRNA Activity in Arabidopsis

文献类型： 外文期刊

第一作者： Wang, Wei

作者： Wang, Wei;Ye, Ruiqiang;Xin, Ying;Fang, Xiaofeng;Li, Chunlian;Shi, Huiqing;Qi, Yijun;Wang, Wei;Wang, Wei;Ye, Ruiqiang;Zhou, Xueping;Xin, Ying;Qi, Yijun

作者机构：

期刊名称：PLANT CELL （ 影响因子：11.277； 五年影响因子：12.061 ）

ISSN： 1040-4651

年卷期： 2011 年 23 卷 10 期

页码：

收录情况： SCI

摘要： As key components in the eukaryotic gene regulatory network, microRNAs (miRNAs) themselves are regulated at the level of both metabolism and activity. To identify factors that modulate miRNA activity, we used an Arabidopsis thaliana transgenic line expressing an artificial miRNA that causes trichome clustering and performed a screen for mutants with compromised miRNA activity (cma mutants) or enhanced miRNA activity (ema mutants). From this screen, we identified two novel mutant alleles of SERRATE, which is known to be required for miRNA biogenesis and dozens of other cma and ema mutants. In this study, we analyzed ema1. SAD2/EMA1 encodes an Importin beta protein. The ema1 mutation had no effects on the accumulation of miRNAs and ARGONAUTE1 (AGO1) or on their cytoplasmic and nuclear distributions. Intriguingly, we found that the miRNA effector complexes purified from ema1 contained a larger amount of miRNAs and displayed elevated mRNA cleavage activities, indicating that EMA1 modulates miRNA activity by influencing the loading of miRNAs into AGO1 complexes. These results implicate EMA1 as a negative regulator of the miRNA pathway and reveal a novel layer of miRNA activity modulation.

分类号： Biochemistry & Molecular Biology; Plant Sciences; Cell Biology

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