Synthesis of partial glycerides rich in alpha-linolenic acid efficiently from silkworm pupa oil with immobilized lipase MAS1-H108A

文献类型： 外文期刊

第一作者： Yue, Haipeng

作者： Yue, Haipeng;He, Shi;Wang, Yonghua;Luo, Ri Ming;Qin, Xiaoli;Yang, Bo;Liao, Sentai;Wang, Weifei

作者机构：

关键词： lipase; glycerolysis; partial glycerides; silkworm pupa oil

期刊名称：FOOD SCIENCE AND TECHNOLOGY （ 影响因子：2.602； 五年影响因子：2.968 ）

ISSN： 0101-2061

年卷期： 2022 年 42 卷

页码：

收录情况： SCI

摘要： In this paper the ability of immobilized lipase MAS1-H108A to prepare alpha -linolenic acid-rich partial glycerides was studied. The effects of different reaction conditions on the yield of Diacylglycerol (DAG) and Monoglyceride (MAG) were investigated, including substrate molar ratio, substrate concentration, temperature and lipase dosage. The highest yield (63.92% of MAG and 30.61% of DAG) was obtained when silkworm pupa oil/glycerol ratio of 1:3, lipase dosage was 50 U/g, substrate concentration was 40% (w/v), at 40 degrees C. Under the optimal conditions, the scale-up (50 times) and single-stage molecular distillation were carried out to separate DAG and MAG, the yield of MAG in the light phase was 99.18%, and the concentration of DAG in the heavy phase was 89.47%, alpha -linolenic acid in MAG and DAG accounted for 28.96% and 28.23%, respectively. The results can be shown that the immobilized lipase MAS1-H108A has excellent potential for enzymatic glycerolysis to prepare functional partial glycerides.

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