Characterization of trichome-specific BAHD acyltransferases involved in acylsugar biosynthesis in Nicotiana tabacum

文献类型： 外文期刊

第一作者： Chang, Aixia

作者： Chang, Aixia;Chen, Biao;Chen, Ming;Qu, Yafang;Yu, Weisong;Sun, Huiqing;Cao, Jianmin;Li, Chenying;Cao, Yanan;Yang, Aiguo;Wang, Yuanying;Hu, Zhongyi;Vanderschuren, Herve;Vasudevan, Kumar;Li, Yangyang;Zhang, Jianye;Shen, Yeming;Vanderschuren, Herve

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关键词： Acylsugar biosynthesis; glandular trichome; Nicotiana tabacum (tobacco); NtASAT1; NtASAT2; plant defence

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：7.298； 五年影响因子：8.291 ）

ISSN： 0022-0957

年卷期： 2022 年 73 卷 12 期

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收录情况： SCI

摘要： ASAT1 and ASAT2 are trichome-specific acyltransferases in tobacco that sequentially catalyse two additive steps of acyl donors to sucrose to produce di-acylsucrose. Glandular trichomes of tobacco (Nicotiana tabacum) produce blends of acylsucroses that contribute to defence against pathogens and herbivorous insects, but the mechanism of assembly of these acylsugars has not yet been determined. In this study, we isolated and characterized two trichome-specific acylsugar acyltransferases that are localized in the endoplasmic reticulum, NtASAT1 and NtASAT2. They sequentially catalyse two additive steps of acyl donors to sucrose to produce di-acylsucrose. Knocking out of NtASAT1 or NtASAT2 resulted in deficiency of acylsucrose; however, there was no effect on acylsugar accumulation in plants overexpressing NtASAT1 or NtASAT2. Genomic analysis and profiling revealed that NtASATs originated from the T subgenome, which is derived from the acylsugar-producing diploid ancestor N. tomentosiformis. Our identification of NtASAT1 and NtASAT2 as enzymes involved in acylsugar assembly in tobacco potentially provides a new approach and target genes for improving crop resistance against pathogens and insects.

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