Transcriptome and proteome profile of jejunum in chickens challenged with Salmonella Typhimurium revealed the effects of dietary bilberry anthocyanin on immune function

文献类型： 外文期刊

第一作者： Zhang, Sheng

作者： Zhang, Sheng;Wang, Qin;Ye, Jinling;Fan, Qiuli;Lin, Xiajing;Gou, Zhongyong;Wang, Yibing;Jiang, Shouqun;Azzam, Mahmoud M.

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关键词： bilberry anthocyanin; Salmonella Typhimurium; immune status; transcriptome; proteome; yellow-feathered chicks

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

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年卷期： 2023 年 14 卷

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收录情况： SCI

摘要： Introduction: The present study investigated the effects of bilberry anthocyanin (BA) on immune function when alleviating Salmonella Typhimurium (S. Typhimurium) infection in chickens.Methods: A total of 180 newly hatched yellow-feathered male chicks were assigned to three groups (CON, SI, and SI + BA). Birds in CON and SI were fed a basal diet, and those in SI + BA were supplemented with 100 mg/kg BA for 18 days. Birds in SI and SI + BA received 0.5 ml suspension of S. Typhimurium (2 x 10(9) CFU/ml) by oral gavage at 14 and 16 days of age, and those in CON received equal volumes of sterile PBS.Results: At day 18, (1) dietary BA alleviated weight loss of chickens caused by S. Typhimurium infection (P < 0.01). (2) Supplementation with BA reduced the relative weight of the bursa of Fabricius (P < 0.01) and jejunal villus height (P < 0.05) and increased the number of goblet cells (P < 0.01) and the expression of MUC2 (P < 0.05) in jejunal mucosa, compared with birds in SI. (3) Supplementation with BA decreased (P < 0.05) the concentration of immunoglobulins and cytokines in plasma (IgA, IL-1 beta, IL-8, and IFN-beta) and jejunal mucosa (IgG, IgM, sIgA, IL-1 beta, IL-6, IL-8, TNF-alpha, IFN-beta, and IFN-gamma) of S. Typhimurium-infected chickens. (4) BA regulated a variety of biological processes, especially the defense response to bacteria and humoral immune response, and suppressed cytokine-cytokine receptor interaction and intestinal immune network for IgA production pathways by downregulating 6 immune-related proteins.Conclusion: In summary, the impaired growth performance and disruption of jejunal morphology caused by S. Typhimurium were alleviated by dietary BA by affecting the expression of immune-related genes and proteins, and signaling pathways are related to immune response associated with immune cytokine receptors and production in jejunum.

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