A chromosome-scale genome assembly of Dasypyrum villosum provides insights into its application as a broad-spectrum disease resistance resource for wheat improvement

文献类型： 外文期刊

第一作者： Zhang, Xu

作者： Zhang, Xu;Wang, Haiyan;Sun, Haojie;Feng, Yilong;Jiao, Chengzhi;Li, Mengli;Song, Xinying;Wang, Tong;Wang, Zongkuan;Yuan, Chunxia;Sun, Li;Zhang, Wenli;Xiao, Jin;Wang, Xiue;Li, Yingbo;Lu, Ruiju

作者机构：

关键词： D; villosum genome; crop wild relative; genome evolution; disease resistance; RNA-seq; MNase hy-persensitivity assay

期刊名称：MOLECULAR PLANT （ 影响因子：27.5； 五年影响因子：22.6 ）

ISSN： 1674-2052

年卷期： 2023 年 16 卷 2 期

页码：

收录情况： SCI

摘要： Dasypyrum villosum is one of the most valuable gene resources in wheat improvement, especially for dis-ease resistance. The mining of favorable genes from D. villosum is frustrated by the lack of a whole genome sequence. In this study, we generated a doubled-haploid line, 91C43DH, using microspore culture and ob-tained a 4.05-GB high-quality, chromosome-scale genome assembly for D. villosum. The assembly con-tains39 727 high-confidence genes, and 85.31% of the sequences are repetitive. Two reciprocal transloca-tion events were detected, and 7VS-4VL is a unique translocation in D. villosum. The prolamin seed storage protein-coding genes were found to be duplicated; in particular, the genes encoding low-molecular-weight glutenin at the Glu-V3 locus were significantly expanded. RNA sequencing (RNA-seq) analysis indicated that, afterBlumeria graminearum f.sp tritici (Bgt) inoculation, there were more upregulated genes involved in the pattern-triggered immunity and effector-triggered immunity defense pathways in D. villosum than in Triticum urartu. MNase hypersensitive sequencing (MH-seq) identified two Bgt-inducible MH sites (MHSs), one in the promoter and one in the 30 terminal region of the powdery mildew resistance (Pm) gene NLR1-V. Each site had two subpeaks and they were termed MHS1 (MHS1.1/1.2) and MHS2 (MHS2.1/2.2). Bgt- inducible MHS2.2 was uniquely present in D. villosum, and MHS1.1 was more inducible in D. villosum than in wheat, suggesting that MHSs may be critical for regulation of NLR1-V expression and plant defense. In summary, this study provides a valuable genome resource for functional genomics studies and wheat-D. villosum introgression breeding. The identified regulatory mechanisms may also be exploited to develop new strategies for enhancing Pm resistance by optimizing gene expression in wheat.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序