Characterization and potential function of 7-dehydrocholesterol reductase (dhcr7) and lathosterol 5-desaturase (sc5d) in Cynoglossus semilaevis sexual size dimorphism

文献类型： 外文期刊

第一作者： Wang, Jialin

作者： Wang, Jialin;Shi, Rui;Yang, Qian;Chen, Zhangfan;Wang, Jiacheng;Gong, Zhihong;Chen, Songlin;Wang, Na;Wang, Jialin;Shi, Rui;Yang, Qian;Wang, Jiacheng;Gong, Zhihong;Chen, Zhangfan;Chen, Songlin;Wang, Na;Chen, Zhangfan;Chen, Songlin;Wang, Na

作者机构：

关键词： Chinese tongue sole ( Cynoglossus semilaevis ); 7-dehydrocholesterol reductase ( dhcr7 ); lathosterol 5-desaturase ( sc5d ); Promoter activity; siRNA; Protein subcellular localization

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2023 年 853 卷

页码：

收录情况： SCI

摘要： The typical sexual size dimorphism (SSD) phenomenon of Chinese tongue sole (Cynoglossus semilaevis) seriously restricts the sustainable development of the fishing industry. Previous transcriptome analysis has found a close relationship between the steroid biosynthesis and C. semilaevis SSD. The 7-dehydrocholesterol reductase (dhcr7) and lathosterol 5-desaturase (sc5d) are two genes in the steroid biosynthesis pathway, playing important roles in lipid synthesis, cellular metabolism, and growth. The present study assessed their roles in the mechanism of C. semilaevis SSD. The quantitative polymerase chain reaction (qPCR) results showed that C. semilaevis dhcr7 was mainly expressed in female livers, and C. semilaevis sc5d was highly expressed in female livers and gonads. Dualluciferase experiment showed that dhcr7 and sc5d promoters had strong transcriptional activity. The transcription factors E2F transcription factor 1 (E2F1), and CCAAT enhancer binding protein alpha (C/EBP alpha) significantly regulated the transcriptional activity of dhcr7 and sc5d promoters, respectively. Furthermore, small interfering RNA (siRNA) knockdown results showed that expression levels of several genes [SREBF chaperone (scap), membrane-bound transcription factor peptidase, site 1 (mbtps1), fatty acid synthase (fasn), sonic hedgehog (shh), bone morphogenetic protein 2b (bmp2b) and AKT serine/threonine kinase 1 (akt1)] were suppressed. Protein subcellular localization results indicated that Dhcr7 and Sc5d were both specifically distributed in the cytoplasm, with colocalization been observed. The present study provides evidence that dhcr7 and sc5d might regulate C. semilaevis sexual size dimorphism by involving in energy homeostasis and cell cycle, or by affecting PI3K-Akt and Shh signaling pathways. The detailed roles of these steroid biosynthesis genes regulating C. semilaevis SSD needed more information.

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