Diversification of three TaSOS1 genes and their roles in sodium exclusion in bread wheat (Triticum aestivum L.)

文献类型： 外文期刊

第一作者： Gao, Zhenxian

作者： Gao, Zhenxian;Cao, Qiao;Gao, Xinmei;Yang, Wenlong;Liu, Dongcheng;Zhang, Aimin;Zhang, Aimin

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关键词： bread wheat; TaSOS1 gene; function difference; Triticum aestivum

期刊名称：JOURNAL OF GENETICS （ 影响因子：1.5； 五年影响因子：1.4 ）

ISSN： 0022-1333

年卷期： 2023 年 102 卷 2 期

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收录情况： SCI

摘要： The ability to exclude sodium from the shoot is a crucial feature of salinity tolerance in bread wheat (Triticum aestivum L.). The plasma membrane sodium/proton exchanger salt-overly-sensitive 1 (SOS1) is a critical Na+ efflux protein in plants. Here, we cloned three homologues of the TaSOS1 gene in bread wheat, designated TaSOS1-A1, TaSOS1-B1 and TaSOS1-D1, respectively, according to the location on group 3 chromosomes 3A, 3B and 3D. Sequence analysis demonstrated that the TaSOS1 deduced protein-contained domains similar to the SOS1 protein, including 12 membrane-spanning regions, a long hydrophilic tail in the C-terminus, the cyclic nucleotide-binding domain, the putative auto-inhibitory domain and the phosphorylation motif. Phylogenetic analysis established the evolutionary relationships between the different copies of this gene in bread wheat and its diploid progenitors, as well as with SOS1 genes from Arabidopsis, rice and Brachypodium distachyon. Analysis of transient TaSOS1-A1::green fluorescent protein expression demonstrated that TaSOS1 was exclusively localized to the plasma membrane. The yeast and Arabidopsis complementary test supported the sodium extrusion function of TaSOS1-A1. Virus-induced gene silencing technology was used to further examine the function of TaSOS1-A1 in bread wheat.

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