CcNAC6 Acts as a Positive Regulator of Secondary Cell Wall Synthesis in Sudan Grass (Sorghum sudanense S.)

文献类型： 外文期刊

第一作者： Huang, Yanzhong

作者： Huang, Yanzhong;Wu, Juanzi;Liu, Zhiwei;Qian, Chen;Zhong, Xiaoxian;Lin, Jianyu;Mao, Zhengfeng

作者机构： Jiangsu Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Saline Alkali Soil Improvement & Utilizat, Inst Anim Sci,Natl Forage Breeding Innovat Base JA, Nanjing 210014, Peoples R China;Nanjing Agr Univ, Natl Ctr Soybean Improvement, Key Lab Biol & Genet & Breeding Soybean, Minist Agr,State Key Lab Crop Genet & Germplasm En, Nanjing 210095, Peoples R China;Nanjing Agr Univ, Coll Agrograssland Sci, Nanjing 210095, Peoples R China

关键词： Sudan grass (Sorghum sudanense S.); secondary cell wall; bioinformatics analysis; CcNAC6; lignin

期刊名称：PLANTS-BASEL （ 2022影响因子：4.5； 五年影响因子：4.8 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 10 期

页码：

收录情况： SCI

摘要： The degree of forage lignification is a key factor affecting its digestibility by ruminants such as cattle and sheep. Sudan grass (Sorghum sudanense S.) is a high-quality sorghum forage, and its lignocellulose is mostly stored in the secondary cell wall. However, the secondary cell wall synthesis mechanism of Sudan grass has not yet been studied in depth. To further study the secondary cell wall synthesis mechanism of Sudan grass using established transcriptome data, this study found that CcNAC6, a homologous gene of Arabidopsis AtSND2, is related to the secondary cell wall synthesis of Sudan grass. Accordingly, we constructed a CcNAC6-overexpressing line of Arabidopsis to investigate the function of the CcNAC6 gene in secondary cell wall synthesis. The results showed that the overexpression of the CcNAC6 gene could significantly increase the lignin content of Arabidopsis. Based on subcellular localization analysis, CcNAC6 is found in the nucleus. In addition, yeast two-hybridization screening showed that CcCP1, associated with secondary cell wall synthesis, can interact with CcNAC6. Therefore, the above results indicate that CcNAC6 has a positive regulatory effect on the secondary cell wall synthesis of Sudan grass, and it is speculated that CcNAC6 may be the main regulator of the secondary cell wall synthesis of Sudan grass through its interaction with another regulatory protein, CcCP1. This study provides a theoretical basis and new genetic resources for the creation of new Sudan grass germplasm with a low lignin content.

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