A novel High-Affinity Anti-Capsaicinoids monoclonal antibody for High-Sensitivity Immunoassays: Experimental and computational validation
文献类型： 外文期刊
第一作者： Wen, Weihuan
作者： Wen, Weihuan;Wang, Zhiruo;Zhao, Yupeng;Liu, Zhiwei;Liang, Yifan;Wang, Yupu;Xiong, Wanqi;Luo, Lin;Xu, Zhenlin;Wen, Weihuan;Li, Xinyu;Wang, Jiang;Talib, Hafiza Shaiza;Hu, Jie;Wang, Jiang;Pan, Fei;Li, Xinyu
作者机构：
关键词： Monoclonal antibody; Hybridoma; Capsaicinoids; Molecular docking; Molecular dynamics simulation; And immunoassay
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）
ISSN： 0026-265X
年卷期： 2025 年 208 卷
页码：
收录情况： SCI
摘要： Capsaicinoids are the indicators of "gutter oil" contamination, and their sensitive detection is essential in edible oil quality control. We have developed HOPE1 monoclonal antibody (mAb), which binds major capsaicinoids with higher affinity than previously described YQQD8 mAb. Two immunoassays based on HOPE1 mAb were developed: an indirect competitive enzyme-linked immunosorbent assay with an IC50 of 121.03 pg/mL (linear range: 40.06-365.71 pg/mL) and a time-resolved fluorescence lateral flow immunoassay, which showed an IC50 of 0.57 ng/mL (linear range: 0.14-2.34 ng/mL). Both assays also yielded satisfactory recovery in spike tests. Further, molecular docking and molecular dynamic simulations of HOPE1 mAb highlighted the significance of hydrophobic interface in antibody-capsaicinoid recognition. Our study unveils a high-affinity mAb against capsaicinoids and its use in detecting gutter oil contamination. It is probably the first report on computational validation of capsaicinoids mAbs and their interaction with the target antigen.
分类号：
- 相关文献
作者其他论文 更多>>
-
Dietary Methionine Restriction Alleviates Cognitive Impairment in Alzheimer's Disease Mice via Sex-Dependent Modulation on Gut Microbiota and Tryptophan Metabolism: A Multiomics Analysis
作者：Zhang, Yuyu;Li, Yiju;Wang, Da;Pang, Jingxi;Sang, Haojie;Xi, Yujia;Liu, Zhigang;Liu, Tianqi;Shi, Lin;Pan, Fei;Li, Yiju;Liu, Zhigang
关键词：Alzheimer's disease; methionine restriction; sexual dimorphism; indole-3-propionic acid; peroxisome proliferator-activated receptor alpha; multiomics
-
Construction of cellulose nanocrystals based rod-like electrostatic nanocomplexes and the digestion characteristics of its stable high internal phase emulsions in vitro
作者：Chen, Hualei;Pan, Fei;Peng, Wenjun;Li, Xiangxin;Fang, Xiaoming;Tian, Wenli
关键词：Aminated sugar beet pectin; Cellulose nanocrystals; Cinnamaldehyde; High internal phase emulsions; Digestion characteristics
-
Screening for Bongkrekic Acid in Food Using a Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay (icELISA)
作者：Wu, Hui-Ling;Chen, Jing;Xia, Qi-Sheng;Zhang, Shi-Wei;Chen, Xue-Jian;Feng, Rong-Hu;Zhang, Gui-Hong;Lai, Xin-Tian;Pan, Fei;Liu, Lei;Tan, Pan
关键词：Bongkrekic acid; hapten; indirect competitive enzyme-linked immunosorbent assay (icELISA); isobongkrekic acid; monoclonal antibody
-
Highly cost-effective wheat starch-stearic acid complexes enabled by microwave processing: Structural properties, anti-digestion, and molecular dynamics simulation
作者：Gao, Tingting;Sun, Da-Wen;Tian, You;Ma, Ji;Gao, Tingting;Sun, Da-Wen;Tian, You;Ma, Ji;Gao, Tingting;Sun, Da-Wen;Tian, You;Ma, Ji;Gao, Tingting;Sun, Da-Wen;Tian, You;Ma, Ji;Sun, Da-Wen;Pan, Fei
关键词：Microwave; Starch-stearic acid complexes; Resistant starch; In vitro digestion; Molecular dynamics simulation
-
Based on theoretical design simultaneous analysis of multiple neonicotinoid pesticides in beeswax by deep eutectic solvents extraction combined with UHPLC-MS/MS
作者：Mu, Guodong;Pan, Fei;Xu, Haitao;Xue, Xiaofeng;Yan, Sha;Jing, Xu
关键词：Beeswax; Neonicotinoid pesticides; Deep eutectic solvent; Conduct or-like screening model for real sol- vents; Molecular dynamics simulation; LC-MS/MS
-
Distinguishing the botanical origins of rare honey through untargeted metabolomics and machine learning interpreting flavonoid profiles
作者：Yan, Sha;Yuan, Yuzhe;Pan, Fei;Mu, Guodong;Xu, Haitao;Xue, Xiaofeng;Yan, Sha
关键词：Botanic origin; Flavonoids; Machine learning; Monofloral honey; Natural deep eutectic solvent; Untargeted metabolomics
-
Genome-Wide Identification and Expression Analysis of the Basic Helix-Loop-Helix (bHLH) Transcription Factor Family in Gastrodia elata
作者：Lan, Shanshan;Lin, Xin;Wei, Maoqiong;Feng, Guangheng;Liu, Hongcheng;Shao, Jinliang;Li, Xinyu;Chen, Zeli;Liu, Zhenhuan
关键词：
Gastrodia elata ; BHLH transcription factor (TF); Gene family identification; Transcriptome; Evolutionary analysis
