Screening for Bongkrekic Acid in Food Using a Monoclonal Antibody-Based Indirect Competitive Enzyme-Linked Immunosorbent Assay (icELISA)

文献类型： 外文期刊

第一作者： Wu, Hui-Ling

作者： Wu, Hui-Ling;Chen, Jing;Xia, Qi-Sheng;Zhang, Shi-Wei;Chen, Xue-Jian;Feng, Rong-Hu;Zhang, Gui-Hong;Lai, Xin-Tian;Pan, Fei;Liu, Lei;Tan, Pan

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关键词： Bongkrekic acid; hapten; indirect competitive enzyme-linked immunosorbent assay (icELISA); isobongkrekic acid; monoclonal antibody

期刊名称：ANALYTICAL LETTERS （ 影响因子：1.8； 五年影响因子：1.8 ）

ISSN： 0003-2719

年卷期： 2025 年 58 卷 11 期

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收录情况： SCI

摘要： Pseudomonas cocovenenans subsp. farinofermentans is a pathogenic bacterium that causes the poisoning of fermented rice products. Its isomeric products, bongkrekic acid (BA) and isobongkrekic acid (IBA), have become popular research topics. In this study, we developed a heterologous-coating strategy for high-sensitivity indirect competitive enzyme-linked immunoassay (icELISA) for BA and IBA using monoclonal antibodies (mAbs). The optimized procedure exhibited an IC50 of 5.49 ng/mL, a limit of detection of 0.99 ng/mL, and a linear range from 2.12 to 14.21 ng/mL. Notably, this method exhibited negligible cross-reactivity to other small toxins, and the recoveries of the samples, including rice noodles, Auricularia auricula, and flat rice noodles, ranged from 92.0%-111.9%, 105.6%-115.0%, and 83.2%-109.0%, respectively, with coefficients of variation below 12%. A comparison of the recoveries of high-performance liquid chromatography-mass spectrometry (HPLC-MS) and icELISA demonstrated that the developed approach provides good accuracy and reliability. Overall, the simple preparation of IBA combined with the suitability of icELISA for high-throughput analysis is efficient and cost-effective, making it suitable for routine screening of food.

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