Overexpression of myo-inositol oxygenase gene GbMIOX8 promotes fiber cell elongation by altering cell wall composition in cotton

文献类型： 外文期刊

第一作者： Wu, Nan

作者： Wu, Nan;Gao, Yu;Wu, Jinhua;Ke, Huifeng;Zhang, Yan;Wang, Guoning;Wu, Liqiang;Zhang, Guiyin;Wang, Xingfen;Ma, Zhiying;Wu, Nan

作者机构：

期刊名称：GENE （ 影响因子：2.4； 五年影响因子：2.8 ）

ISSN： 0378-1119

年卷期： 2025 年 951 卷

页码：

收录情况： SCI

摘要： Cell elongation is an important process during cotton fiber development, ultimately determining the length of mature fibers. Myo-inositol oxygenase (MIOX) pathway provides pivotal precursors for the synthesis of noncellulosic polysaccharides in plant cell walls. However, the role of MIOX gene in cotton fiber development has not been reported. Here, we hypothesized that Gossypium barbadense MIOX gene GbMIOX8 (GbM_D05G1480.1) could regulate fiber length by modulating cell wall composition. To test this hypothesis, we characterized the functional properties of GbMIOX8. GbMIOX8 preferentially expressed during fiber initiation and elongation in cotton and encodes non-secretory protein targeted to the cytoplasm. Overexpression of GbMIOX8 afforded transgenic A. thaliana significantly longer leaf trichomes, as well as longer hypocotyl cells compared to the wild type, with increases of at least 11 % and up to 23 %. We further overexpressed GbMIOX8 in cotton and found that transgenic cotton displayed fiber length that was increased by an average of 1.61 mm in the T1 generation and 1.93 mm in the T2 generation, respectively. Similar to Arabidopsis, transgenic cotton exhibited at least a threefold increase in myo-inositol oxygenase activity and content, boosting glucuronic acid production and reducing inositol. Furthermore, pectin and cellulose contents rose in transgenic cottons, with average rises of 19 % and 38 % respectively, indicating enhanced biosynthesis of these two cell wall components. These results revealed that GbMIOX8 played an important role in the elongation of plant cells by altering cell wall components and could be valuable for cotton fiber quality improvement.

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