Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space

文献类型： 外文期刊

第一作者： Zhang, Feng

作者： Zhang, Feng;Gao, Yu;Ren, Enxi;Fang, Ling;Yang, Weijuan;Zhang, Liaoyuan;Wang, Zongwen;Zhang, Feng;Gao, Yu;Ren, Enxi;Fang, Ling;Yang, Weijuan;Zhang, Liaoyuan;Wang, Zongwen;Fang, Ling

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关键词： Paper-based analytical device; Colorimetric biosensor; Colorimeter; The CIELab color space; Gold nanobipyramids-deposited membrane

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）

ISSN： 0039-9140

年卷期： 2025 年 281 卷

页码：

收录情况： SCI

摘要： On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au(3+)etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-deposited nylon membrane embedded within a portable and temperature-controlled paper-based analytical device. This system, coupled with a colorimeter-based quantitative method, enables the development of a practical paper-based multicolor sensor (PMS) for on-site quantitative detection of three common OPs (paraoxon, dichlorvos, and trichlorfon). In the absence of OPs, AChE hydrolyzes acetylthiocholine to thiocholine, which reduces Au(3+)to A(u+). The presence of OPs inhibits AChE activity, thereby preserving Au3+ to etch AuNBPs on nylon membranes, accompanied by multicolor changes. These color changes can be simply quantified by measuring the a* parameter of the CIELab color space using a portable colorimeter. Under optimal conditions, the PMS displayed eight OPs-corresponding color changes with a minimum detectable concentration of 1.0-10 mu g/L (visual observation) and limits of detection of 0.8-7.2 mu g/L (colorimeter) and 0.2-3.4 mu g/L (UV-vis spectrometry). The PMS successfully determined the OPs in vegetable and rice samples with recoveries of 89.0-109 % and RSDs (n = 5) of <6 %. These results were consistent with those obtained using the HPLC-MS method. The PMS demonstrates excellent portability, AuNBPs stability, detection sensitivity, and reproducibility, making it a promising tool for the on-site quantitative detection of OPs residues in food. Furthermore, the paper-based etching system coupled with the colorimeter-based quantitative method provides a valuable reference to develop practical PMSs for various targets in diverse fields.

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