Transcriptome sequence reveal the roles of MaGME777 and MabHLH770 in tolerance in Musa acuminata

文献类型： 外文期刊

第一作者： Wei, Yuchen

作者： Wei, Yuchen;Zhang, Feng;Dong, Huizhen;Huang, Xiaosan;Li, Yaoyao;Bi, Fangcheng;Dou, Tongxin;Li, Yaoyao;Bi, Fangcheng;Dou, Tongxin;Li, Yaoyao;Bi, Fangcheng;Dou, Tongxin;Zhang, Feng;Dong, Huizhen;Huang, Xiaosan;Shi, Jingfang;Xie, Xueyi

作者机构：

关键词： Cavendish banana; Drought stress; RNA-Seq; WGCNA; BHLH

期刊名称：PLANT SCIENCE （ 影响因子：4.1； 五年影响因子：5.1 ）

ISSN： 0168-9452

年卷期： 2025 年 356 卷

页码：

收录情况： SCI

摘要： Banana, a globally cultivated fruit, faces significant constraints in distribution and sustainable production due to drought stress. This study investigated drought tolerance in Cavendish bananas using RNA-seq time-course analysis and molecular biology experiments. Plants were subjected to dehydration treatments, and physiological indicators such as electrolyte leakage, proline content, malonaldehyde, peroxidase activity, and hydrogen peroxide content were assessed. RNA-Seq and qRT-PCR were used to analyze transcriptional changes under drought. Weighted gene co-expression network (WGCNA) analysis identified thousands of differentially expressed genes (DEGs) at different time points, with a core set of 2660 DEGs consistently identified. KEGG enrichment analysis revealed MaGME777, a glycolysis/gluconeogenesis gene, as a potential drought resistance regulator. Virus-mediated gene silencing (VIGS) of MaGME777 reduced drought tolerance in bananas. Yeast onehybrid (Y1H) and luciferase reporter assays demonstrated that the transcription factor MabHLH770 directly binds and activates the MaGME777 promoter. VIGS downregulation of MabHLH770 also reduced drought tolerance. In conclusion, this study revealed that MabHLH770 is a positive regulator of drought stress, by targeting MaGME777 promoter and activating their expression to enhance drought tolerance. These findings provide a foundation for developing drought-resistant banana cultivars through molecular breeding approaches.

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