Changes in cell wall characteristics and expression of MiERF12, MiERF109-like, and MiERF113 during mango softening

文献类型： 外文期刊

第一作者： Long, Yuhan

作者： Long, Yuhan;Xie, Lihong;Yi, Ping;Huang, Min;Gan, Ting;Li, Li;Fan, Zhenmei;Cheng, Qiuyu;Long, Yuhan;Xie, Lihong;Sun, Jian;Fan, Zhenmei;Yi, Ping;Sun, Jian;Huang, Fang;Li, Li;Dong, Hao;Wei, Qi

作者机构：

关键词： Mango fruit; Ripening; Softening; Ethylene response factor; Cell wall metabolism

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.8； 五年影响因子：7.5 ）

ISSN： 0925-5214

年卷期： 2025 年 227 卷

页码：

收录情况： SCI

摘要： Mango fruit softening is the primary reason for the reduction in shelf life and the subsequent decrease in market value. In this study, we investigated the roles of ethylene response factor (ERF) in regulating mango fruit softening in response to 1-methylcyclopropene (1-MCP) and ethylene (ETH) treatments was investigated. The action mechanism of the cellular microstructure, key structural components of the cell wall, cell wall metabolizing enzymes, and the ERF were evaluated. Results showed that mango softening was induced by ETH and inhibited by 1-MCP. ETH treatment caused cellular microstructural changes, including cell wall thinning and distortion, while 1-MCP treatment preserved cell wall integrity. Biochemical and molecular assays indicated that 1-MCP and ETH regulated fruit softening by altering cell wall polysaccharide fractions, cell wall degrading enzyme activities (pectate lyase, pectin methylesterase, (3-galactosidase, cellulase) and their gene expression (MiPLY8, MiPME3, Mi beta-CAL, MiPG2). Meanwhile, ETH strongly induced the expression of MiERF12, MiERF021-like, MiERF109-like, and MiERF113. Virus-induced gene silencing (VIGS) results indicated MiERF109-like and MiERF113 promoted mango fruit softening, whereas MiERF12 inhibited softening. These findings emphasize that MiERF12, MiERF109like and MiERF113 could play an important role in regulating postharvest ripening and softening of mango, and clarify the potential association of ERFs with cell wall metabolism and structure in mango.

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