The addition of vermiculite reduced antibiotic resistance genes during composting: Novel insights based on reducing host bacteria abundance and inhibiting plasmid-mediated conjugative transfer

文献类型： 外文期刊

第一作者： Sun, Yifan

作者： Sun, Yifan;Zhao, Zixuan;Gao, Zixuan;Hu, Tao;An, Lu;Gu, Jie;Wang, Xiaojuan;Gu, Jie;Wang, Xiaojuan;Sun, Jian;Guo, Honghong

作者机构：

关键词： Antibiotic resistance gene; Host bacteria; Mobile genetic elements; Conjugative transfer; Vermiculite

期刊名称：JOURNAL OF ENVIRONMENTAL MANAGEMENT （ 影响因子：8.4； 五年影响因子：8.6 ）

ISSN： 0301-4797

年卷期： 2025 年 373 卷

页码：

收录情况： SCI

摘要： Antibiotic resistance genes (ARGs) are prevalent in raw materials used for composting. The utilization of ecofriendly materials for the removal of ARGs is regarded as an economically effective method. Therefore, this study focused on the impact of incorporating different proportions of vermiculite (0% (CK), 5% (T1), and 10% (T2)) on the dynamics of ARGs during composting. In comparison to CK, the total absolute abundances of ARGs decreased by 14.17% and 31.52% in T1 and T2, respectively. Potential human pathogenic bacteria, including Acinetobacter, Corynebacterium, and Lactobacillus, were identified as core hosts of ARGs. The addition of vermiculite effectively inhibited proliferation of ARG hosts by extending the thermophilic phase of composting and reducing bioavailable copper concentrations. Incorporation of vermiculite decreased the absolute abundances of transposons and integrons, such as intI1 and Tn916/1545, which were significantly positively correlated with most ARGs. Adding vermiculite simultaneously enhanced the removal of common environmental plasmids (e.g., Inc.P, Inc.W), and downregulated expression of genes associated with bacterial conjugation and plasmid replication (e.g., trBbp, trfAp), thereby inhibiting the dissemination of ARGs. Taken together, this study provides novel insights that the incorporation of vermiculite can effectively enhance the reduction rate of ARGs during composting by reducing the host of ARGs and inhibiting their dissemination.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序