Rapid detection of feline parvovirus using RAA-CRISPR/Cas12a-based lateral flow strip and fluorescence
文献类型： 外文期刊
第一作者： Chen, Han
作者： Chen, Han;Guo, Jie;Meng, Xiangshu;Yao, Mengfan;He, Longbin;Nie, Xiaoxuan;Xu, Han;Liu, Chao;Sun, Jian;Zhang, Jianlou;Wang, Jianke;Zhang, Hailing;Sun, Jian;Wang, Fei;Sun, Yuelong;Jiang, Zhong;He, Yanliang
作者机构：
关键词： CRISPR/Cas12a; detection; feline; parvovirus; RAA; lateral flow strip
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.2 ）
ISSN：
年卷期： 2025 年 16 卷
页码：
收录情况： SCI
摘要： Feline parvovirus (FPV) causes severe gastroenteritis and leukopenia in cats, with high morbidity and mortality, necessitating a rapid and effective antigen diagnostic test with high sensitivity and specificity. In this study, a diagnostic platform based on a combination of Recombinase-Aided Amplification (RAA) and CRISPR/Cas12a was established for detecting FPV. Cas12a recombinant protein was purified using Nickel-Nitriloacetic Acid resin after heterologous expression in Escherichia coli. The results of RAA-CRISPR/Cas12a can be detected with a fluorescence reader or lateral flow strips (LFS) for on-site detection. The RAA-CRISPR/Cas12a-LFS had a detection limit of 2.1 x 100 copies of recombinant plasmids per reaction, compared with 2.1 x 103 copies for conventional PCR analysis. Furthermore, no cross-reactivity was observed for the RAA-CRISPR/Cas12a assay with feline coronavirus, feline herpesvirus, and feline calicivirus, demonstrating reasonable specificity. Additionally, 43 cat fecal samples with suspected clinical signs were assayed with RAA-CRISPR/Cas12a-LFS and conventional PCR in parallel. The RAA-CRISPR/Cas12a-LFS showed a 100% coincident rate with PCR. In summary, a novel, visual, sensitive, and specific detection assay based on RAA and CRISPR/Cas12a was developed for FPV.
分类号：
- 相关文献
作者其他论文 更多>>
-
A naphthimide based fluorescent probe for the detection of thiophenols and its application in actual water and food
作者：Zhang, Di;Duan, Ran;Zhou, Xiaohua;Shi, Jiacheng;Chen, He;Guo, Jie;Li, Man;Wang, Tieliang;Wu, Xujin;Ma, Zhiwei;Shi, Jiacheng
关键词：Thiophenol; Fluorescence probe; Off-on; Water; Food
-
Unveiling the aromatic diversity of Fermented mango juices through 40 plant-derived Lactiplantibacillus plantarum
作者：Ma, Tianyu;Wang, Chunguang;Zhang, Xinyue;Zhu, Yuxuan;Liu, Jiani;Zhu, Baoqing;Sun, Jian;Ye, Dongqing
关键词：Lactiplantibacillus plantarum; Mango juice; Acid-producing diversity; Aroma-producing diversity; Napping
-
Pyraclostrobin repeated treatment altered the degradation behavior in soil and negatively affected soil bacterial communities and functions
作者：Han, Lingxi;Wang, Yiran;Wang, Yajie;Xu, Han;Liu, Mingyu;Nie, Jiyun;Wang, Qianwen;Huang, Bin
关键词：Pyraclostrobin; Apple orchard soils; Degradation characteristics; Soil bacterial communities; Soil nitrogen cycling
-
Integrative analysis of transcriptome and metabolism reveals functional roles of redox homeostasis in low light and salt combined stress in Leymus chinensis
作者：Li, Jikai;Zhang, Hailing;Shang, Chen;Han, Weibo;Li, Jikai;Fang, Suyang;Iqbal, Zubair;Huang, Kai;Meng, Xiangshen;Dai, Muyuan;Lu, Zhiheng;Guo, Bingnan;Qu, Mingnan
关键词：Sheepgrass; Transcriptome; Photorespiration; ROS; Carbon metabolism; Antioxidant; TCA
-
The addition of vermiculite reduced antibiotic resistance genes during composting: Novel insights based on reducing host bacteria abundance and inhibiting plasmid-mediated conjugative transfer
作者：Sun, Yifan;Zhao, Zixuan;Gao, Zixuan;Hu, Tao;An, Lu;Gu, Jie;Wang, Xiaojuan;Gu, Jie;Wang, Xiaojuan;Sun, Jian;Guo, Honghong
关键词：Antibiotic resistance gene; Host bacteria; Mobile genetic elements; Conjugative transfer; Vermiculite
-
Integration of volatile and non-volatile metabolite profile, and in vitro digestion reveals the differences between different preparation methods on physico-chemical and biological properties of Gastrodia elata
作者：Li, Shi;He, Xiahong;Zhang, Xuechun;Wang, Zhenxing;Kong, Kin Weng;Xie, Jianhua;Sun, Jian
关键词：Gastrodia elata Blume; Processing and drying method; In vitro simulated digestion; Biological activity; Sensory assessment; Metabolite
-
Identification of two effective sex-specific DNA markers in silver arowana ( Osteoglossum bicirrhosum)
作者：Liu, Yi;Liu, Chao;Yang, Yexin;Wang, Yuanyuan;Mu, Xidong;Sun, Jinhui;Yang, Yexin
关键词：Osteoglossidae; Silver arowana; Sex-specific markers; ZW sex-determination
