Drug Resistance Modulation of Dairy MRSA through Berberine, Artesunate and Quercetin in Combination with β-Lactams
文献类型： 外文期刊
第一作者： Ahmad, Saad
作者： Ahmad, Saad;Jianxi, Li;Aqib, Amjad Islam;Ghafoor, Muzafar;Shoaib, Muhammad;ul Haq, Shahbaz;Ataya, Farid Shokry
作者机构：
关键词： Key Artesunate; Berberine; Quercetin; S. aureus; Drug resistance; Resistance modulation
期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：3.8； 五年影响因子：2.2 ）
ISSN： 0253-8318
年卷期： 2024 年 44 卷 2 期
页码：
收录情况： SCI
摘要： The continued use of antibiotics is bringing extensive resistance in methicillinresistant Staphylococcus aureus (MRSA) of dairy animals and demands inclusion of non-antibiotic sources in treatment protocols. The current study aims to explore antibacterial potential of berberine (BR), artesunate (AR), and quercetin (QT) and extent of their resistance modulation capacity in combination with beta-lactam antibiotics against MRSA. For this study, a total of 674 milk samples from dairy cows were collected for isolation of MRSA and thereafter phytochemicals (drugs) alone and in combination with beta-lactams (penicillin, oxacillin, cefoxitin, ampicillin) were evaluated against MRSA. The synergy testing showed all synergistic effects except AR with penicillin which was additive effect. The synergism of antibiotics and phytochemicals was further confirmed by time-kill assay. The killing kinetics revealed a zero bacterial count at 16h of incubation in all combinations. Additionally, the killing synergy showed a significant decline i.e. less than 60% in case of BR in combination with oxacillin and penicillin at the initial 2h of incubation followed by AR and QT in combination with ampicillin. The study thus revealed potential antibacterial effects of berberine, artesunate, and quercetin along with significant resistance modulation in terms of highly synergistic combinations with antibiotics focused on novel insights into alternatives to antimicrobials to pave the road for antimicrobial stewardship against ailments like mastitis.
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