Molecular mapping and characterization of QBp.caas-3BL for black point resistance in wheat (Triticum aestivum L.)

文献类型： 外文期刊

第一作者： Liu, Cuihe

作者： Liu, Cuihe;Song, Jie;Liu, Siyang;Xu, Dengan;Tian, Xiuling;Bian, Yingjie;Dong, Yachao;Wang, Fengju;Wang, Rongge;He, Zhonghu;Xia, Xianchun;Cao, Shuanghe;Liu, Jingdong;Wang, Rongge;He, Zhonghu

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关键词： Black point; Candidate gene prediction; Fine mapping; Marker-assisted selection; Wheat

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2021 年 134 卷 10 期

页码：

收录情况： SCI

摘要： Key message We fine-mapped QBp.caas-3BL for black point resistance in an interval of 1.7 Mb containing five high-confidence annotated genes and developed a KASP marker suitable for selection of QBp.caas-3BL. Wheat black point, which occurs in most wheat-growing regions of the world, is detrimental to grain appearance, processing and nutrient quality. Mining and characterization of genetic loci for black point resistance are helpful for breeding resistant wheat cultivars. We previously identified a major QTL QBp.caas-3BL in a recombinant inbred line (RIL) population of Linmai 2/Zhong 892 across five environments. Here we confirmed the QTL in two additional environments. The genetic region of QBp.caas-3BL was enriched with newly developed markers. Using four sets of near isogenic lines, QBp.caas-3BL was narrowed down to a physical interval of approximately 1.7 Mb, including five annotated genes according to IWGSC reference genome. TraesCS3B02G404300, TraesCS3B02G404600 and TraesCS3B02G404700 were predicted as candidate genes based on the analyses of sequence polymorphisms and differential expression. We also converted a SNP of TraesCS3B02G404700 into a breeding-applicable KASP marker and verified its efficacy for marker-assisted breeding in a panel of germplasm. The findings not only lay a foundation for map-based cloning of QBp.caas-3BL but also provide a useful marker for selection of resistant cultivars genotypes in wheat breeding.

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