On the use of EST-SSR to analysis the genetic diversity of segetale rye from China

文献类型： 外文期刊

第一作者： Che, Yonghe

作者： Che, Yonghe;Yang, Yanping;Wei, Lai;Yang, Yunjie;Wen, Xiaolei;Guo, Juan;Che, Yonghe;Yang, Yanping;Wei, Lai;Yang, Yunjie;Wen, Xiaolei;Guo, Juan;Yang, Xinming;Li, Xiuquan;Liu, Weihua;Li, Lihui

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关键词： S; cereale subsp; segetale; EST-SSR; Cluster analysis; Genetic diversity

期刊名称：CEREAL RESEARCH COMMUNICATIONS （ 影响因子：1.6； 五年影响因子：1.4 ）

ISSN： 0133-3720

年卷期： 2023 年

页码：

收录情况： SCI

摘要： A weedy rye, Secale cereale subsp. segetale found in Xinjiang, China, possess strong stress tolerance, tillering capacity and many useful traits. The study aims to better understand the genetic diversity of segetale rye from China at the molecular level. The genetic diversity of 92 populations (bulked samples) and further 168 plants (individual samples from 14 populations) in the segetale rye were analyzed using 47 polymorphic EST-SSR (expressed sequence tags derived microsatellite) molecular markers. A total of 211 bands were amplified by 47 EST-SSR primers in the 92 populations. Among them, there were 190 polymorphic bands, with an average of 4.04 bands per primer and an average polymorphic ratio of 88.4%. The variation range of PIC (polymorphic information content) was 0.16-0.94 with the average 0.75, indicating abundant genetic diversity of weedy rye populations. The clustering analysis of 92 populations based on Nei's genetic distance showed that their average genetic distance was near 0.35, and 92 populations from different regions of Xinjiang were relatively scattered, generally close genetic distance between populations in the same region. Similar rule was found between individual plants in same population using clustering and principal coordinate analysis (PCoA). To 168 individual plants from 14 populations, analysis found that the average genetic differentiation among populations was 0.143, and the genetic diversity of the segetale rye populations mainly existed within populations (85.7%). Abundant genetic diversity was found within different regions and populations of segetale rye from China. The research provides a theoretical basis on the application of elite genes of segetale rye to the genetic improvement of rye and wheat.

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