Unraveling Morphological, Physiological, and Transcriptomic Alterations Underlying the Formation of Little Leaves in Phytoplasma-Infected Sweet Cherry Trees

文献类型： 外文期刊

第一作者： Tan, Yue

作者： Tan, Yue;Xu, Li;Zhu, Min;Wei, Hairong;Zhao, Yan;Wei, Wei

作者机构：

关键词： arrested cell cycle; impaired ribosome biogenesis; little leaf; premature leaf senescence; sweet cherry virescence phytoplasma; witches' broom

期刊名称：PLANT DISEASE （ 影响因子：4.4； 五年影响因子：4.8 ）

ISSN： 0191-2917

年卷期： 2025 年 109 卷 2 期

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收录情况： SCI

摘要： Phytoplasmas are minute phytopathogenic bacteria that induce excessive vegetative growth, known as witches' broom (WB), in many infected plant species during the later stages of infection. The WB structure is characterized by densely clustered little (small) leaves, which are frequently accompanied by chlorosis (yellowing). The mechanisms behind the formation of little leaves within WB structures (LL-WB) are poorly understood. To address this gap, the LL-WB formation was extensively studied using sweet cherry virescence (SCV) phytoplasma-infected sweet cherry plants. Based on morphological examinations, signs of premature leaf senescence were observed in LL-WB samples, including reduced leaf size, chlorosis, and alterations in shape. Subsequent physiological analyses indicated decreased sucrose and glucose levels and changes in hormone concentrations in LL-WB samples. Additionally, the transcriptomic analysis revealed impaired ribosome biogenesis and DNA replication. As an essential process in protein production, the compromised ribosome biogenesis and the inhibited DNA replication led to cell cycle arrest, thus affecting leaf morphogenesis and further plant development. Moreover, the expression of marker genes involved in premature leaf senescence was significantly altered. These results indicate a complicated interplay between the development of leaves, premature leaf senescence, and pathogen-induced stress responses in SCV phytoplasma-infected sweet cherry trees. The results of this study provide insight into understanding the underlying molecular mechanisms driving the formation of little leaves and interactions between plants and pathogens. The findings might help control phytoplasma diseases in sweet cherry cultivation.

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