Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant

文献类型： 外文期刊

第一作者： Latif, Muhammad Faizan

作者： Latif, Muhammad Faizan;Tan, Jingquan;Zhang, Wang;Yang, Wenxuan;Zhuang, Tinghui;Lu, Wenlong;Qiu, Yanglin;Du, Xinying;Zhuang, Xinjian;Zhou, Tong;Yin, Jinlong;Xu, Kai;Zhou, Tong;Kundu, Jiban K.;Kundu, Jiban K.

作者机构：

关键词： MicroRNA; Potyvirus; SMV; P1 gene; Transgenic; Virus resistance

期刊名称：TRANSGENIC RESEARCH （ 影响因子：3.0； 五年影响因子：2.7 ）

ISSN： 0962-8819

年卷期： 2024 年

页码：

收录情况： SCI

摘要： RNA silencing is an innate immune mechanism of plants against invasion by viral pathogens. Artificial microRNA (amiRNA) can be engineered to specifically induce RNA silencing against viruses in transgenic plants and has great potential for disease control. Here, we describe the development and application of amiRNA-based technology to induce resistance to soybean mosaic virus (SMV), a plant virus with a positive-sense single-stranded RNA genome. We have shown that the amiRNA targeting the SMV P1 coding region has the highest antiviral activity than those targeting other SMV genes in a transient amiRNA expression assay. We transformed the gene encoding the P1-targeting amiRNA and obtained stable transgenic Nicotiana benthamiana lines (amiR-P1-3-1-2-1 and amiR-P1-4-1-2-1). Our results have demonstrated the efficient suppression of SMV infection in the P1-targeting amiRNA transgenic plants in an expression level-dependent manner. In particular, the amiR-P1-3-1-2-1 transgenic plant showed high expression of amiR-P1 and low SMV accumulation after being challenged with SMV. Thus, a transgenic approach utilizing the amiRNA technology appears to be effective in generating resistance to SMV.

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