Modulation of flavonoid-fatty acid crosstalk underlies light-shading enhanced α-linolenic acid biosynthesis in oilseed tree peony (Paeonia ostii 'Feng Dan')

文献类型： 外文期刊

第一作者： Han, Chenjing

作者： Han, Chenjing;Wang, Qi;Li, Jian;Liu, Zhifang;Mu, Yanjuan;Sun, Tao;Lu, Yizeng;Wang, Zhiwei

作者机构：

关键词： Metabolomics; Transcriptomics; Co-expression analysis; Substrate competition; Carbon flux redirecting

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 233 卷

页码：

收录情况： SCI

摘要： During seed filling stage, excessive light and heat stress significantly impair photosynthetic efficiency, leading to compromised seed yield and quality. Our previous findings revealed that light-shading significantly elevated alpha-linolenic acid (ALA) levels in tree peony seeds. However, the environmental signal transduction pathways coordinating unsaturated fatty acid biosynthesis are still unclear. Black low density polyethylene nets were utilized from 28 days after pollination (DAP) until harvest. Integrated metabolomic and transcriptomic analyses were performed at three critical time points (28, 56, and 98 DAP) to identify key metabolites, genes, transcription factors, signaling molecules, and regulatory networks. Comprehensive profiling detected 706 metabolites and 42,775 genes in total. Differentially expressed metabolites (DEMs) were predominantly enriched in "flavonoid biosynthesis". A substantial number of differentially expressed genes (DEGs) were significantly enriched in "alpha-linolenic acid metabolism" and "flavonoid biosynthesis" pathways. Coordinated regulatory interactions were observed between key DEMs (linoleic acid, ALA, gamma-linolenic acid, naringenin chalcone, and naringenin) and core DEGs (SAD, FAD2, FAD3, CHS, and CHI). Under light-shading, transcription factors gene families (MYB, FAR1, and bHLH) exhibited marked alterations, concomitant with upregulation of the phytohormone jasmonic acid (JA). Flavonoid and fatty acid biosynthesis pathways exhibit competitive substrate allocation dynamics under shared precursor pools. Light-shading alleviated high-temperature and high-light stress, thereby reducing reactive oxygen species (ROS) generation and lowering the demand for antioxidant flavonoids. This adaptive response was transcriptionally coordinated through phytohormone-mediated signaling cascades and transcriptional factor networks, redirecting carbon flux from flavonoid biosynthesis toward unsaturated fatty acid biosynthesis via precursor reallocation mechanisms. The current investigation elucidated the photoregulatory mechanism underlying ALA enhancement in tree peony seeds under light-shading treatment, while also establishing a theoretical framework for understanding the molecular regulatory network driving PUFAs biosynthesis in oilseed crops through environmental modulation.

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