Microbial inoculants and garbage fermentation liquid reduced root-knot nematode disease and As uptake in Panax quinquefolium cultivation by modulating rhizosphere microbiota community

文献类型： 外文期刊

第一作者： Cao, Pei

作者： Cao, Pei;Wei, Xuemin;Wang, Gang;Chen, Xiaochen;Han, Jianping;Li, Yuan

作者机构： Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Med Plant Dev, Key Lab Chinese Med Resources Conservat, Natl Adm Tradit Chinese Med Peoples Republ China, Beijing 100193, Peoples R China;Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100193, Peoples R China

关键词： garbage fermentation liquid; heavy metal; microbial inoculants; Panax quinquefolium L; nematodes disease

期刊名称：CHINESE HERBAL MEDICINES （ 2022影响因子：3.8； 五年影响因子：2.7 ）

ISSN： 1674-6384

年卷期： 2022 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Objective: To find a suitable ecological cultivation measure to solve the problem of root-knot nematode disease of Panax quinquefolium (Panacis Quinquefolii Radix) and the heavy metals accumulating in its roots. Methods: Three-year-old P. quinquefolium was treated with four different combinations of microbial inoculant (MI) and garbage fermentation liquid (GFL) [the joint application of 'TuXiu' MI and Fifty potassium MI (TF), the combination use of 'No. 10 MI and Fifty potassium MI (NF), 'Gulefeng' poly-gamma-glutamic acid MI (PGA), GFL], and the untreated control (CK). Here, high-throughput sequencing, ICP-MS and UPLC were employed to systematically characterize changes of microbial diversity and structure composition, heavy metals (As, Cd and Pb) content and ginsenoside content among different treatments. Results: The results revealed that different MIs and GFL could increase the root dry weight of P. quinquefolium, PGA enhanced it by 83.24%, followed by GFL (49.93%), meanwhile, PGA and GFL were able to lessen root-knot nematode disease incidence by 57.25% and 64.35%. The treatment of PGA and GFL can also effectively reduce heavy metals in roots. The As content in GFL and PGA was decreased by 52.17% and 43.48% respectively, while the Cd and Pb contents of GFL and PGA was decreased somewhat. Additionally, the content of total ginsenosides was increased by 42.14% and 42.07%, in response to TF and NF, respectively. Our metagenomic analysis showed that the relative abundance of particular soil microbial community members related to the biocontrol of root-knot nematode disease and plant pathogen (i.e., Chaetomium in NF, Xylari in GFL, and Microascus in PGA), heavy metal bioremediation (Hyphomacrobium in PGA and Xylaria in GFL), and nitrogen fixation (Nordella and Nitrospira in TF) was significantly increased; notably, potential harmful microflora, such as Plectosaphaerella and Rhizobacter, were more abundant in the control group. Conclusion: MI and GFL could improve the quality of P. quinquefolium by modifying its rhizosphere microbial community structure and composition, both of them are beneficial to the development of ecological cultivation of P. quinquefolium. (C) 2021 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V.

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