A novel study of brain microvascular endothelial cells induced by astrocyte conditioned medium for constructing blood brain barrier model in vitro: A promising tool for meningitis of teleost

文献类型： 外文期刊

第一作者： Chen, Yanghui

作者： Chen, Yanghui;Luo, Junliang;Li, Zixin;Huang, Yu;Cai, Jia;Jiang, Dongneng;Jian, Jichang;Wang, Bei;Chen, Yanghui;Luo, Junliang;Li, Zixin;Huang, Yu;Cai, Jia;Jiang, Dongneng;Jian, Jichang;Wang, Bei;Li, Yuan;Zhang, Defeng;Qiang, Jun

作者机构：

关键词： Nile tilapia; Endothelial cell line; BBB; Astrocyte-conditioned medium (ACM)

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 146 卷

页码：

收录情况： SCI

摘要： The blood-brain barrier (BBB) is mainly composed of specialized endothelial cells, which can resist harmful substances, transport nutrients, and maintain the stability of the brain environment. In this study, an endothelial cell line from tilapia (Oreochromis niloticus) named TVEC-01 was successfully established. During the earlier establishment phase of the cell line, the TVEC-01 cells were persistently exposed to an astrocyte-conditioned medium (ACM). TVEC-01 cells were identified as an endothelial cell line. TVEC-01 cells retained the multiple functions of endothelial cells and were capable of performing various experiments in vitro. Furthermore, TVEC01 cells efficiently expressed BBB-related tight junctions and key efflux transporters. From the results of the qRTPCR, we found that the TVEC-01 cell line did not gradually lose BBB characteristics after persistent and repetitive passages, which was different from the vast majority of immortalized endothelial cells. The results showed that ACM induced up-regulation of the expression levels of multiple BBB-related genes in TVEC-01 cells. We confirmed that Streptococcus agalactiae was capable of invading the TVEC-01 cells and initiating a series of immune responses, which provided a theoretical basis for S. agalactiae to break through the BBB of teleost through the transcellular traversal pathway. In summary, we have successfully constructed an endothelial cell line of teleost, named TVEC-01, which can be used in many experiments in vitro and even for constructing BBB in vitro. Moreover, it was confirmed that S. agalactiae broke through the BBB of teleost through the transcellular traversal pathway and caused meningitis.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序