The Bombyx mori G protein β subunit 1 ( BmGN fi 1 ) gene inhibits BmNPV infection

文献类型： 外文期刊

第一作者： Yu, Linyuan

作者： Yu, Linyuan;Ling, Chenyu;Li, Yizhu;Guo, Huiduo;Xu, Anying;Qian, Heying;Li, Gang;Guo, Huiduo;Xu, Anying;Qian, Heying;Li, Gang

作者机构：

关键词： Bombyx mori; BmNPV; GNfi1; Apoptosis; PI3K-AKT

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：3.4； 五年影响因子：3.1 ）

ISSN： 0022-2011

年卷期： 2024 年 204 卷

页码：

收录情况： SCI

摘要： G protein beta subunit 1 ( GNfi1 ) has several functions, including cell growth regulation, the control of second messenger levels, and ion channel switching. Previous transcriptome analyses in our laboratory have shown that BmGNfi1 transcription is reduced following infection with Bombyx mori nucleopolyhedrovirus (BmNPV), but it is unknown what role this gene may have in the host response to BmNPV infection. In this study, the BmGNfi1 gene was cloned using the RACE method. After BmNPV infection, BmGNfi1 was downregulated in Baiyu strains in tissues such as the hemolymph and midgut. Indirect immunofluorescence showed that BmGN beta 1 was localized to the cytoplasm. We further constructed a BmGNfi1 -pIZ/V5-His-mCherry overexpression plasmid and designed siRNA to evaluate the role of BmGNfi1 in host response to infection. The results showed that BmGNfi1 overexpression inhibited BmNPV proliferation, while knockdown of BmGNfi1 was correlated with increased BmNPV proliferation. The siRNA-mediated reduction of BmGNfi1 was correlated with an increase in BmNPV infection of BmN cells, increased BmNPV vp39 transcription, and reduced survival time of BmNPV-infected B. mori . Overexpression of BmGNfi1 in BmN cells was also correlated with apoptosis and a modification in transcript levels of genes involved in host response to BmNPV infection ( PI3K, AKT , Bmp53 , BmFOXO , Caspase-1, Bmp21 , BmPKN and BmCREB ), suggesting that BmGNfi1 may influence the apoptotic host response of infected B. mori through the PI3K-AKT pathway. This study provides potential targets and theoretical support for breeding BmNPV-resistant silkworm varieties.

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