Transcriptome analysis used to identify and characterize odorant binding proteins in Agasicles hygrophila (Coleoptera: Chryspmelidae)

文献类型： 外文期刊

第一作者： Dong, Changhong

作者： Dong, Changhong;Zhu, Daohong;Dong, Changhong;Huang, Cong;Ning, Xiaoyu;Liu, Bo;Qiao, Xi;Qian, Wanqiang;Wan, Fanghao;Huang, Cong;Wan, Fanghao;Ning, Xiaoyu

作者机构：

关键词： Agasicles hygrophila; odorant-binding protein; transcriptome analysis; egg; first instar larvae

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：2.2； 五年影响因子：2.0 ）

ISSN：

年卷期： 2023 年 23 卷 5 期

页码：

收录情况： SCI

摘要： The transcriptomes of Agasicles hygrophila eggs and first instar larvae were analyzed to explore the olfactory mechanism of larval behavior. The analysis resulted in 135,359 unigenes and the identification of 38 odorant-binding proteins (OBPs), including 23 Minus-C OBPs, 8 Plus-C OBPs, and 7 Classic OBPs. Further analysis of differentially expressed genes (DEGs) revealed 10 DEG OBPs, with 5 (AhygOBP5, AhygOBP9, AhygOBP12, AhygOBP15 and AhygOBP36) up-regulated in first instar larvae. Verification of expression patterns of these 5 AhygOBPs using qPCR showed that AhygOBP9 and AhygOBP36 were mainly expressed in the adult stage with gradually increasing expression in the larval stage. AhygOBP5, AhygOBP12, and AhygOBP15 were not expressed in eggs and pupae, and their expression in larvae and adults showed no clear pattern. These 5 AhygOBPs may play an olfactory role in larval behavior, providing a basis for further investigation of their specific functions and clarifying the olfactory mechanism of A. hygrophila.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序