DNA methylase 1 influences temperature responses and development in the invasive pest Tuta absoluta

文献类型： 外文期刊

第一作者： Tang, Yanhong

作者： Tang, Yanhong;Zhang, Huifang;Zhu, Huanqing;Bi, Siyan;Wang, Xiaodi;Ji, Shunxia;Ji, Jianhang;Huang, Cong;Zhang, Guifen;Yang, Nianwan;Wan, Fanghao;Lue, Zhichuang;Liu, Wanxue;Tang, Yanhong;Zhu, Huanqing;Ma, Dongfang;Yang, Nianwan

作者机构：

关键词： DNA methylation; Dnmt1; larvae growth; larval development; RNA interference; temperature response; Tuta absoluta

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：2.6； 五年影响因子：2.9 ）

ISSN： 0962-1075

年卷期： 2024 年

页码：

收录情况： SCI

摘要： DNA methylase 1 (Dnmt1) is an important regulatory factor associated with biochemical signals required for insect development. It responds to changes in the environment and triggers phenotypic plasticity. Meanwhile, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)-a destructive invasive pest-can rapidly invade and adapt to different habitats; however, the role of Dnmt1 in this organism has not been elucidated. Accordingly, this study investigates the mechanism(s) underlying the rapid adaptation of Tuta absoluta to temperature stress. Potential regulatory genes were screened via RNAi (RNA interference), and the DNA methylase in Tuta absoluta was cloned by RACE (Rapid amplification of cDNA ends). TaDnmt1 was identified as a potential regulatory gene via bioinformatics; its expression was evaluated in response to temperature stress and during different development stages using real-time polymerase chain reaction. Results revealed that TaDnmt1 participates in hot/cold tolerance, temperature preference and larval development. The full-length cDNA sequence of TaDnmt1 is 3765 bp and encodes a 1254 kDa protein with typical Dnmt1 node-conserved structural features and six conserved DNA-binding active motifs. Moreover, TaDnmt1 expression is significantly altered by temperature stress treatments and within different development stages. Hence, TaDnmt1 likely contributes to temperature responses and organismal development. Furthermore, after treating with double-stranded RNA and exposing Tuta absoluta to 35 degrees C heat shock or -12 degrees C cold shock for 1 h, the survival rate significantly decreases; the preferred temperature is 2 degrees C lower than that of the control group. In addition, the epidermal segments become enlarged and irregularly folded while the surface dries up. This results in a significant increase in larval mortality (57%) and a decrease in pupation (49.3%) and eclosion (50.9%) rates. Hence, TaDnmt1 contributes to temperature stress responses and temperature perception, as well as organismal growth and development, via DNA methylation regulation. These findings suggest that the rapid geographic expansion of T absoluta has been closely associated with TaDnmt1-mediated temperature tolerance. This study advances the research on 'thermos Dnmt' and provides a potential target for RNAi-driven regulation of Tuta absoluta.

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