Biomineralized ZIF-8 Encapsulating SOD from Hydrogenobacter Thermophilus: Maintaining Activity in the Intestine and Alleviating Intestinal Oxidative Stress

文献类型： 外文期刊

第一作者： Li, Yuying

作者： Li, Yuying;Chen, Weihua;Jiang, Yayun;Ge, Qianqian;Liu, Jinping;Wang, Mansheng;Hou, Zhenping;Shi, Pengjun;Chen, Weihua;Yin, Jie;Xia, Siting;Jiang, Yayun;Ge, Qianqian;Liu, Jinping;Bai, Yingguo

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关键词： antioxidants; hydrogenobacter thermophiles; stability; superoxide dismutase; ZIF-8

期刊名称：SMALL （ 影响因子：12.1； 五年影响因子：12.5 ）

ISSN： 1613-6810

年卷期： 2024 年 20 卷 50 期

页码：

收录情况： SCI

摘要： Oxidative stress is a major factor leading to inflammation and disease occurrence, and superoxide dismutase (SOD) is a crucial antioxidative metalloenzyme capable of alleviating oxidative stress. In this study, a novel thermostable SOD gene is obtained from the Hydrogenobacter thermophilus strain (HtSOD), transformed and efficiently expressed in Escherichia coli with an activity of 3438 U mg(-1), exhibiting excellent thermal stability suitable for scalable production. However, the activity of HtSOD is reduced to less than 10% under the acidic environment. To address the acid resistance and gastrointestinal stability issues, a biomimetic mineralization approach is employed to encapsulate HtSOD within the ZIF-8 (HtSOD@ZIF-8). Gastrointestinal simulation results show that HtSOD@ZIF-8 maintained 70% activity in simulated gastric fluid for 2 h, subsequently recovering to 97% activity in simulated intestinal fluid. Cell and in vivo experiments indicated that HtSOD@ZIF-8 exhibited no cytotoxicity and do not impair growth performance. Furthermore, HtSOD@ZIF-8 increased the relative abundance of beneficial microbiota such as Dubosiella and Alistipes, mitigated oxonic stress and intestinal injury by reducing mitochondrial and total reactive oxygen species (ROS) levels in diquat-induced. Together, HtSOD@ZIF-8 maintains and elucidates activity in the intestine and biocompatibility, providing insights into alleviating oxidative stress in hosts and paving the way for scalable production.

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