Engineering globins for efficient biodegradation of malachite green: two case studies of myoglobin and neuroglobin

文献类型： 外文期刊

第一作者： Liu, Jiao

作者： Liu, Jiao;Wang, Xiao-Juan;Lin, Ying-Wu;Xu, Jia-Kun;Yuan, Hong;Tan, Xiang-Shi;Wang, Xiao-Juan;Gao, Shu-Qin;Wen, Ge-Bo;Lin, Ying-Wu

作者机构： Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China;Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries,Minist Ag, Lab Marine Drugs & Byproducts Pilot Natl Lab Mari, Qingdao 266071, Peoples R China;Fudan Univ, Inst Biomed Sci, Dept Chem, Shanghai 200433, Peoples R China;Univ South China, Lab Prot Struct & Funct, Hengyang 421001, Peoples R China

期刊名称：RSC ADVANCES （ 2021影响因子：4.036； 五年影响因子：3.748 ）

ISSN：

年卷期： 2022 年 12 卷 29 期

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收录情况： SCI

摘要： Malachite green (MG)-contaminated wastewater resulting from industrialization causes a global problem because of its toxicity and widespread usage. Compared with traditional physical and chemical approaches, biodegradation provides a new route for the degradation of MG. As promising candidates for native enzymes, artificial enzymes have received tremendous attention for potential applications due to unlimited possibilities based on precise design. In this study, we rationally engineered artificial enzymes based on myoglobin (Mb) and neuroglobin (Ngb). We introduced an aspartic acid (H64D mutation) in the heme pocket of Mb. A distal histidine (F43H mutation) was further introduced into H64D Mb to obtain a double mutant of F43H/H64D Mb. Moreover, we used A15C/H64D Ngb as designed recently for comparison studies. The H64D Mb, F43H/H64D Mb, and A15C/H64D Ngb were found to catalyze MG degradation efficiently, with activities much higher than those of native enzymes, such as dye-decolorizing peroxidase and laccase (83-205-fold). The crystal structure of H64D Mb was solved and the interactions of MG and H64D Mb and A15C/H64D Ngb were investigated by using both experimental and molecular docking studies. The biodegradation products of MG were also revealed by ESI-MS analysis. Therefore, these artificial enzymes have potential applications in the biodegradation of MG in textile industries and fisheries.

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